PPP1R15B

Protein identityUniProt · HPA

UniProt accession

Q5SWA1

Gene

PPP1R15B

Ensembl

ENSG00000158615

Chromosome

1

Canonical length

713 aa

Protein class

Disease related genes, Human disease related genes, Predicted intracellular proteins

Subcellular location

Golgi apparatus

OverviewNCBI Gene

This gene encodes a protein phosphatase I-interacting protein that promotes the dephosphorylation of eukaryotic translation initiation factor 2A to regulate translation under conditions of cellular stress. The transcribed messenger RNA contains two upstream open reading frames (ORFs) that repress translation of the main protein coding ORF under normal conditions, while the protein coding ORF is expressed at high levels in response to stress. Continual translation of the mRNA under conditions of eukaryotic translation initiation factor 2A inactivation is thought to create a feedback loop for reactivation of the gene during recovery from stress. In addition, it has been shown that this protein plays a role in membrane traffic that is independent of translation and that it is required for exocytosis from erythroleukemia cells. Allelic variants of this gene are associated with microcephaly, short stature, and impaired glucose metabolism. [provided by RefSeq, Feb 2016]

Canonical amino-acid sequenceUniProt

713 residues, UniProt reviewed canonical sequence.

>Q5SWA1|PPP1R15B
     1  MEPGTGGSRK RLGPRAGFRF WPPFFPRRSQ AGSSKFPTPL GPENSGNPTL LSSAQPETRV
    61  SYWTKLLSQL LAPLPGLLQK VLIWSQLFGG MFPTRWLDFA GVYSALRALK GREKPAAPTA
   121  QKSLSSLQLD SSDPSVTSPL DWLEEGIHWQ YSPPDLKLEL KAKGSALDPA AQAFLLEQQL
   181  WGVELLPSSL QSRLYSNREL GSSPSGPLNI QRIDNFSVVS YLLNPSYLDC FPRLEVSYQN
   241  SDGNSEVVGF QTLTPESSCL REDHCHPQPL SAELIPASWQ GCPPLSTEGL PEIHHLRMKR
   301  LEFLQQASKG QDLPTPDQDN GYHSLEEEHS LLRMDPKHCR DNPTQFVPAA GDIPGNTQES
   361  TEEKIELLTT EVPLALEEES PSEGCPSSEI PMEKEPGEGR ISVVDYSYLE GDLPISARPA
   421  CSNKLIDYIL GGASSDLETS SDPEGEDWDE EAEDDGFDSD SSLSDSDLEQ DPEGLHLWNS
   481  FCSVDPYNPQ NFTATIQTAA RIVPEEPSDS EKDLSGKSDL ENSSQSGSLP ETPEHSSGEE
   541  DDWESSADEA ESLKLWNSFC NSDDPYNPLN FKAPFQTSGE NEKGCRDSKT PSESIVAISE
   601  CHTLLSCKVQ LLGSQESECP DSVQRDVLSG GRHTHVKRKK VTFLEEVTEY YISGDEDRKG
   661  PWEEFARDGC RFQKRIQETE DAIGYCLTFE HRERMFNRLQ GTCFKGLNVL KQC

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PPP1R15B can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.67

Highest tissue expression

111 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about PPP1R15B.

Disease | AllUniProt

Conditions PPP1R15B is implicated in, by any mechanism.

• Microcephaly, short stature, and impaired glucose metabolism 2 (MSSGM2) MIM:616817

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.51

gnomAD pLI

0.15

gnomAD missense Z

-1.35

DepMap mean gene effect

-0.82

DepMap dependency class

common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 10% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• ER overload response
• negative regulation of PERK-mediated unfolded protein response
• negative regulation of protein phosphorylation
• regulation of translation
• response to endoplasmic reticulum stress
• response to hydrogen peroxide

Molecular functions

• eukaryotic initiation factor eIF2 binding
• molecular adaptor activity
• protein phosphatase 1 binding
• protein phosphatase regulator activity

Cellular components

• endoplasmic reticulum
• protein phosphatase type 1 complex

Protein domainsUniProt · Pfam · InterPro

• Protein phosphatase 1, regulatory subunit 15A/B, C-terminal
• Protein Phosphatase 1 Regulatory Subunit 15
• Phosphatase-1 catalytic subunit binding region
• Protein phosphatase 1, regulatory subunit 15B, N-terminal
• eIF2-alpha phosphatase phosphorylation constitutive repressor

KeywordsUniProt

• Diabetes mellitus
• Dwarfism
• Intellectual disability
• Phosphoprotein
• Translation regulation

InteractionsUniProt · HPA

Protein binding partners of PPP1R15B in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• PP1
• PPP1CA
• PPP1CC