PPEF2

Protein identityUniProt · HPA

UniProt accession

O14830

Gene

PPEF2

Ensembl

ENSG00000156194

Chromosome

4

Canonical length

753 aa

Protein class

Enzymes, Predicted intracellular proteins

OverviewNCBI Gene

This gene encodes a member of the serine/threonine protein phosphatase with EF-hand motif family. The protein contains a protein phosphatase catalytic domain, and at least two EF-hand calcium-binding motifs in its C terminus. Although its substrate(s) is unknown, the encoded protein, which is expressed specifically in photoreceptors and the pineal, has been suggested to play a role in the visual system. This gene shares high sequence similarity with the Drosophila retinal degeneration C (rdgC) gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

753 residues, UniProt reviewed canonical sequence.

>O14830|PPEF2
     1  MGSGTSTQHH FAFQNAERAF KAAALIQRWY RRYVARLEMR RRCTWSIFQS IEYAGQQDQV
    61  KLHDFFSYLM DHFIPSSHND RDFLTRIFTE DRFAQDSEMK KCSDYESIEV PDSYTGPRLS
   121  FPLLPDHATA LVEAFRLKQQ LHARYVLNLL YETKKHLVQL PNINRVSTCY SEEITVCGDL
   181  HGQLDDLIFI FYKNGLPSPE RSYVFNGDFV DRGKDSVEIL MILFAFMLVY PKEFHLNRGN
   241  HEDHMVNLRY GFTKEVMNKY KVHGKEILRT LQDVFCWLPL ATLIDEKVLI LHGGVSDITD
   301  LELLDKIERS KIVSTMRCKT RQKSEKQMEE KRRANQKSSA QGPIPWFLPE SRSLPSSPLR
   361  LGSYKAQKTS RSSSIPCSGS LDGRELSRQV RSSVELELER CRQQAGLLVT GEKEEPSRSA
   421  SEADSEAGEL RKPTQEEWRQ VVDILWSDPM AQEGCKANTI RGGGCYFGPD VTQQLLQKYN
   481  MQFLIRSHEC KPEGYEFCHN RKVLTIFSAS NYYEVGSNRG AYVKLGPALT PHIVQYQANK
   541  VTHTLTMRQR ISRVEESALR ALREKLFAHS SDLLSEFKKH DADKVGLITL SDWAAAVESV
   601  LHLGLPWRML RPQLVNSSAD NMLEYKSWLK NLAKEQLSRE NIQSSLLETL YRNRSNLETI
   661  FRIIDSDHSG FISLDEFRQT WKLFSSHMNI DITDDCICDL ARSIDFNKDG HIDINEFLEA
   721  FRLVEKSCPE GDASECPQAT NAKDSGCSSP GAH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PPEF2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.32

Highest tissue expression

221 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.97

gnomAD pLI

0

gnomAD missense Z

0.04

DepMap mean gene effect

-0.1

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cellular response to hydrogen peroxide
• detection of stimulus involved in sensory perception
• negative regulation of apoptotic signaling pathway
• negative regulation of MAPK cascade
• protein dephosphorylation
• visual perception

Molecular functions

• calcium ion binding
• Hsp70 protein binding
• Hsp90 protein binding
• iron ion binding
• manganese ion binding
• mitogen-activated protein kinase kinase kinase binding
• protein serine/threonine kinase inhibitor activity
• protein serine/threonine phosphatase activity

Cellular components

• cytosol
• nucleus
• photoreceptor inner segment
• photoreceptor outer segment

Protein domainsUniProt · Pfam · InterPro

• EF-hand domain
• Calcineurin-like, phosphoesterase domain
• Serine/threonine-specific protein phosphatase/bis(5-nucleosyl)-tetraphosphatase
• EF-hand domain pair
• Serine/threonine-protein phosphatase with EF-hands
• PPP domain
• EF-Hand 1, calcium-binding site
• Metallo-dependent phosphatase-like
• Protein Phosphatase PPP
• Calcineurin-like phosphoesterase
• PPP5 TPR repeat region
• EF-hand domain pair

KeywordsUniProt

• Calcium
• Cell projection
• Cilium
• Cytoplasm
• Hydrolase
• Manganese
• Metal-binding
• Protein phosphatase
• Repeat
• Sensory transduction
• Vision