POU5F2
POU domain, class 5, transcription factor 2
Also known as: FLJ25680, PO5F2_HUMAN, SPRM-1
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- Q8N7G0
- Gene
- POU5F2
- Ensembl
- ENSG00000248483
- Chromosome
- 5
- Canonical length
- 328 aa
- Protein class
- Predicted intracellular proteins, Transcription factors
- Subcellular location
- Nucleoplasm
OverviewNCBI Gene
Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Predicted to be located in chromatin. [provided by Alliance of Genome Resources, Jul 2025]
Canonical amino-acid sequenceUniProt
328 residues, UniProt reviewed canonical sequence.
>Q8N7G0|POU5F2
1 MAGHRPSNHF CPLPGSGGGG PRGPMPLRVD TLTWLSTQAA PGRVMVWPAV RPGICPGPDV
61 WRIPLGPLPH EFRGWIAPCR PRLGASEAGD WLRRPSEGAL PGPYIALRSI PKLPPPEDIS
121 GILKELQQLA KELRQKRLSL GYSQADVGIA VGALFGKVLS QTTICRFEAQ QLSVANMWKL
181 RPLLKKWLKE VEAENLLGLC KMEMILQQSG KWRRASRERR IGNSLEKFFQ RCPKPTPQQI
241 SHIAGCLQLQ KDVVRVWFYN RSKMGSRPTN DASPREIVGT AGPPCPGAPV CFHLGLGLPV
301 DIPHYTRLYS AGVAHSSAPA TTLGLLRFLocalizationUniProt · AlphaFold · HPA
Whether an antibody against POU5F2 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.56
- Highest tissue expression
- 1.8 nTPM
Expression across tissuesHPA
Tissue
- testis: 1.8 nTPM
- retina: 0.9 nTPM
- bone marrow: 0.4 nTPM
- skin: 0.4 nTPM
- breast: 0.2 nTPM
- cerebral cortex: 0.2 nTPM
Single-cell type
- paneth cells: 3.9 nCPM
- hematopoietic stem cells: 0.5 nCPM
- epididymal basal cells: 0.3 nCPM
- late primary spermatocytes: 0.3 nCPM
- mast cells: 0.3 nCPM
- mesothelial cells: 0.3 nCPM
Immune cell
- neutrophil: 0.4 nTPM
- basophil: 0.3 nTPM
- naive B-cell: 0.3 nTPM
- NK-cell: 0.2 nTPM
- T-reg: 0.2 nTPM
- classical monocyte: 0.1 nTPM
Brain region
- cerebellum: 9.4 nTPM
- white matter: 9 nTPM
- medulla oblongata: 8 nTPM
- pons: 8 nTPM
- thalamus: 8 nTPM
- cerebral cortex: 7.7 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 1.8
- gnomAD pLI
- 0
- gnomAD missense Z
- 0.41
- DepMap mean gene effect
- -0.09
- DepMap dependency class
- selective
Cancer expressionTCGA
Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).
OntologyGO
Biological processes
Molecular functions
- DNA-binding transcription factor activity, RNA polymerase II-specific
- RNA polymerase II cis-regulatory region sequence-specific DNA binding
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads POU5F2 as an antibody target. Whether an autoantibody or antibody against POU5F2 could matter depends on whether native POU5F2 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
POU5F2 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label POU5F2 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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