POU3F1

Protein identityUniProt · HPA

UniProt accession

Q03052

Gene

POU3F1

Ensembl

ENSG00000185668

Chromosome

1

Canonical length

451 aa

Protein class

Predicted intracellular proteins, Transcription factors

Subcellular location

Nucleoplasm

OverviewNCBI Gene

Enables sequence-specific double-stranded DNA binding activity. Predicted to be involved in negative regulation of transcription by RNA polymerase II; positive regulation of DNA-templated transcription; and positive regulation of gene expression. Predicted to act upstream of or within keratinocyte differentiation; nervous system development; and positive regulation of transcription by RNA polymerase II. Located in nucleoplasm. [provided by Alliance of Genome Resources, Apr 2025]

Canonical amino-acid sequenceUniProt

451 residues, UniProt reviewed canonical sequence.

>Q03052|POU3F1
     1  MATTAQYLPR GPGGGAGGTG PLMHPDAAAA AAAAAAAERL HAGAAYREVQ KLMHHEWLGA
    61  GAGHPVGLAH PQWLPTGGGG GGDWAGGPHL EHGKAGGGGT GRADDGGGGG GFHARLVHQG
   121  AAHAGAAWAQ GSTAHHLGPA MSPSPGASGG HQPQPLGLYA QAAYPGGGGG GLAGMLAAGG
   181  GGAGPGLHHA LHEDGHEAQL EPSPPPHLGA HGHAHGHAHA GGLHAAAAHL HPGAGGGGSS
   241  VGEHSDEDAP SSDDLEQFAK QFKQRRIKLG FTQADVGLAL GTLYGNVFSQ TTICRFEALQ
   301  LSFKNMCKLK PLLNKWLEET DSSSGSPTNL DKIAAQGRKR KKRTSIEVGV KGALESHFLK
   361  CPKPSAHEIT GLADSLQLEK EVVRVWFCNR RQKEKRMTPA AGAGHPPMDD VYAPGELGPG
   421  GGGASPPSAP PPPPPAALHH HHHHTLPGSV Q

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against POU3F1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.6

Highest tissue expression

31 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.71

gnomAD pLI

0.56

gnomAD missense Z

2.54

DepMap mean gene effect

0

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• axon ensheathment
• forebrain development
• keratinocyte differentiation
• myelination in peripheral nervous system
• positive regulation of DNA-templated transcription
• positive regulation of gene expression
• regulation of transcription by RNA polymerase II

Molecular functions

• DNA-binding transcription factor activity
• DNA-binding transcription factor activity, RNA polymerase II-specific
• RNA polymerase II cis-regulatory region sequence-specific DNA binding
• sequence-specific DNA binding
• sequence-specific double-stranded DNA binding

Cellular components

• chromatin
• nucleoplasm
• nucleus
• transcription regulator complex

Protein domainsUniProt · Pfam · InterPro

• POU-specific domain
• Homeodomain
• Homedomain-like superfamily
• Lambda repressor-like, DNA-binding domain superfamily
• POU domain
• POU-domain transcription factor, class 3
• Homeobox, conserved site
• POU domain transcription factor
• Homeodomain
• Pou domain - N-terminal to homeobox domain

KeywordsUniProt

• Activator
• DNA-binding
• Homeobox
• Nucleus
• Repressor
• Transcription
• Transcription regulation

InteractionsUniProt · HPA

Protein binding partners of POU3F1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• HMGB2