Seroatlas · Human Serome Atlas

POU2AF1

POU domain class 2-associating factor 1

Also known as: BOB1, OBF1, OBF1_HUMAN, OCA-B

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q16633
Gene: POU2AF1
Ensembl: ENSG00000110777
Chromosome: 11
Canonical length: 256 aa
Protein class: Cancer-related genes, Disease related genes, Plasma proteins, Predicted intracellular proteins

OverviewNCBI Gene

Enables transcription coactivator activity. Involved in positive regulation of transcription by RNA polymerase II. Part of RNA polymerase II transcription regulator complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

256 residues, UniProt reviewed canonical sequence.

>Q16633|POU2AF1
     1  MLWQKPTAPE QAPAPARPYQ GVRVKEPVKE LLRRKRGHAS SGAAPAPTAV VLPHQPLATY
    61  TTVGPSCLDM EGSVSAVTEE AALCAGWLSQ PTPATLQPLA PWTPYTEYVP HEAVSCPYSA
   121  DMYVQPVCPS YTVVGPSSVL TYASPPLITN VTTRSSATPA VGPPLEGPEH QAPLTYFPWP
   181  QPLSTLPTST LQYQPPAPAL PGPQFVQLPI SIPEPVLQDM EDPRRAASSL TIDKLLLEEE
   241  DSDAYALNHT LSVEGF

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against POU2AF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.71
Highest tissue expression: 112 nTPM

Expression across tissuesHPA

Tissue

tonsil: 112 nTPM
lymph node: 93 nTPM
spleen: 86 nTPM
appendix: 46 nTPM
small intestine: 41 nTPM
stomach: 38 nTPM

Single-cell type

plasma cells: 538 nCPM
choroid plexus epithelial cells: 377 nCPM
respiratory ciliated cells: 183 nCPM
b-cells: 158 nCPM
gastric chief cells: 106 nCPM
ependymal cells: 102 nCPM

Immune cell

memory B-cell: 34 nTPM
naive B-cell: 24 nTPM
basophil: 3.9 nTPM
neutrophil: 1.6 nTPM
T-reg: 1.2 nTPM
total PBMC: 1.2 nTPM

Brain region

choroid plexus: 58 nTPM
pons: 30 nTPM
white matter: 30 nTPM
cerebral cortex: 29 nTPM
midbrain: 28 nTPM
medulla oblongata: 28 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.57
gnomAD pLI: 0.57
gnomAD missense Z: 0.95
DepMap mean gene effect: -0.08
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

RNA polymerase II transcription regulator complex

Protein domainsUniProt · Pfam · InterPro

OCA domain
POU, class 2, associating factor 1
POU domain, class 2, associating factor 1

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of POU2AF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads POU2AF1 as an antibody target. Whether an autoantibody or antibody against POU2AF1 could matter depends on whether native POU2AF1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

POU2AF1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label POU2AF1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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