PIR

Protein identityUniProt · HPA

UniProt accession

O00625

Gene

PIR

Ensembl

ENSG00000087842

Chromosome

X

Canonical length

290 aa

Protein class

Enzymes, Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Nuclear bodies,Cytosol

OverviewNCBI Gene

This gene encodes a member of the cupin superfamily. The encoded protein is an Fe(II)-containing nuclear protein expressed in all tissues of the body and concentrated within dot-like subnuclear structures. Interactions with nuclear factor I/CCAAT box transcription factor as well as B cell lymphoma 3-encoded oncoprotein suggest the encoded protein may act as a transcriptional cofactor and be involved in the regulation of DNA transcription and replication. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

290 residues, UniProt reviewed canonical sequence.

>O00625|PIR
     1  MGSSKKVTLS VLSREQSEGV GARVRRSIGR PELKNLDPFL LFDEFKGGRP GGFPDHPHRG
    61  FETVSYLLEG GSMAHEDFCG HTGKMNPGDL QWMTAGRGIL HAEMPCSEEP AHGLQLWVNL
   121  RSSEKMVEPQ YQELKSEEIP KPSKDGVTVA VISGEALGIK SKVYTRTPTL YLDFKLDPGA
   181  KHSQPIPKGW TSFIYTISGD VYIGPDDAQQ KIEPHHTAVL GEGDSVQVEN KDPKRSHFVL
   241  IAGEPLREPV IQHGPFVMNT NEEISQAILD FRNAKNGFER AKTWKSKIGN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PIR can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.22

Highest tissue expression

37 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.8

gnomAD pLI

0

gnomAD missense Z

-0.4

DepMap mean gene effect

0.12

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• digestion
• monocyte differentiation
• transcription by RNA polymerase II

Molecular functions

• metal ion binding
• transcription coregulator activity
• quercetin 2,3-dioxygenase activity

Cellular components

• cytoplasm
• cytosol
• nuclear body
• nucleoplasm
• nucleus

Protein domainsUniProt · Pfam · InterPro

• RmlC-like cupin domain superfamily
• RmlC-like jelly roll fold
• Pirin, N-terminal domain
• Pirin, C-terminal domain
• Pirin
• Pirin
• Pirin C-terminal cupin domain

KeywordsUniProt

• Cytoplasm
• Dioxygenase
• Iron
• Metal-binding
• Nucleus
• Oxidoreductase
• Transcription
• Transcription regulation

InteractionsUniProt · HPA

Protein binding partners of PIR in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• BCL3