Seroatlas · Human Serome Atlas

PHRF1

PHD and RING finger domain-containing protein 1

Also known as: KIAA1542, PHRF1_HUMAN, PPP1R125, RNF221

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9P1Y6
Gene: PHRF1
Ensembl: ENSG00000070047
Chromosome: 11
Canonical length: 1649 aa
Protein class: Predicted intracellular proteins

OverviewNCBI Gene

Predicted to enable RNA polymerase binding activity. Predicted to be involved in protein ubiquitination. Located in membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

1649 residues, UniProt reviewed canonical sequence.

>Q9P1Y6|PHRF1
     1  MDDDSLDELV ARSPGPDGHP QVGPADPAGD FEESSVGSSG DSGDDSDSEH GDGTDGEDEG
    61  ASEEEDLEDR SGSEDSEDDG ETLLEVAGTQ GKLEAAGSFN SDDDAESCPI CLNAFRDQAV
   121  GTPENCAHYF CLDCIVEWSK NANSCPVDRT LFKCICIRAQ FGGKILRKIP VENTKASEEE
   181  EDPTFCEVCG RSDREDRLLL CDGCDAGYHM ECLDPPLQEV PVDEWFCPEC AAPGVVLAAD
   241  AGPVSEEEVS LLLADVVPTT SRLRPRAGRT RAIARTRQSE RVRATVNRNR ISTARRVQHT
   301  PGRLGSSLLD EAIEAVATGL STAVYQRPLT PRTPARRKRK TRRRKKVPGR KKTPSGPSAK
   361  SKSSATRSKK RQHRVKKRRG KKVKSEATTR SRIARTLGLR RPVHSSCIPS VLKPVEPSLG
   421  LLRADIGAAS LSLFGDPYEL DPFDSSEELS ANPLSPLSAK RRALSRSALQ SHQPVARPVS
   481  VGLSRRRLPA AVPEPDLEEE PVPDLLGSIL SGQSLLMLGS SDVIIHRDGS LSAKRAAPVS
   541  FQRNSGSLSR GEEGFKGCLQ PRALPSGSPA QGPSGNRPQS TGLSCQGRSR TPARTAGAPV
   601  RLDLPAAPGA VQARNLSNGS VPGFRQSHSP WFNGTNKHTL PLASAASKIS SRDSKPPCRS
   661  VVPGPPLKPA PRRTDISELP RIPKIRRDDG GGRRDAAPAH GQSIEIPSAC ISRLTGREGT
   721  GQPGRGTRAE SEASSRVPRE PGVHTGSSRP PAPSSHGSLA PLGPSRGKGV GSTFESFRIN
   781  IPGNMAHSSQ LSSPGFCNTF RPVDDKEQRK ENPSPLFSIK KTKQLRSEVY DPSDPTGSDS
   841  SAPGSSPERS GPGLLPSEIT RTISINSPKA QTVQAVRCVT SYTVESIFGT EPEPPLGPSS
   901  AMSKLRGAVA AEGASDTERE EPTESQGLAA RLRRPSPPEP WDEEDGASCS TFFGSEERTV
   961  TCVTVVEPEA PPSPDVLQAA THRVVELRPP SRSRSTSSSR SRKKAKRKRV SREHGRTRSG
  1021  TRSESRDRSS RSASPSVGEE RPRRQRSKAK SRRSSSDRSS SRERAKRKKA KDKSREHRRG
  1081  PWGHSRRTSR SRSGSPGSSS YEHYESRKKK KRRSASRPRG RECSPTSSLE RLCRHKHQRE
  1141  RSHERPDRKE SVAWPRDRRK RRSRSPSSEH RAREHRRPRS REKWPQTRSH SPERKGAVRE
  1201  ASPAPLAQGE PGREDLPTRL PALGEAHVSP EVATADKAPL QAPPVLEVAA ECEPDDLDLD
  1261  YGDSVEAGHV FDDFSSDAVF IQLDDMSSPP SPESTDSSPE RDFPLKPALP PASLAVAAIQ
  1321  REVSLMHDED PSQPPPLPEG TQEPHLLRPD AAEKAEAPSS PDVAPAGKED SPSASGRVQE
  1381  AARPEEVVSQ TPLLRSRALV KRVTWNLQES ESSAPAEDRA PRAPLHRPQK PREGAWDMED
  1441  VAPTGVRQVF SELPFPSHVL PEPGFPDTDP SQVYSPGLPP APAQPSSIPP CALVSQPTVQ
  1501  FILQGSLPLV GCGAAQTLAP VPAALTPASE PASQATAASN SEEKTPAPRL AAEKTKKEEY
  1561  MKKLHMQERA VEEVKLAIKP FYQKREVTKE EYKDILRKAV QKICHSKSGE INPVKVANLV
  1621  KAYVDKYRHM RRHKKPEAGE EPPTQGAEG

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PHRF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Unknown
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.65
Highest tissue expression: 26 nTPM

Expression across tissuesHPA

Tissue

liver: 26 nTPM
skin: 21 nTPM
cerebellum: 21 nTPM
esophagus: 18 nTPM
skeletal muscle: 18 nTPM
testis: 17 nTPM

Single-cell type

proximal tubule cells: 32 nCPM
renal collecting duct intercalated cells: 28 nCPM
renal connecting tubule cells: 28 nCPM
podocytes: 27 nCPM
distal convoluted tubule cells: 25 nCPM
loop of henle epithelial cells: 23 nCPM

Immune cell

memory CD8 T-cell: 0.5 nTPM
neutrophil: 0.5 nTPM
memory CD4 T-cell: 0.4 nTPM
naive B-cell: 0.4 nTPM
naive CD4 T-cell: 0.4 nTPM
plasmacytoid DC: 0.4 nTPM

Brain region

cerebral cortex: 6.5 nTPM
thalamus: 4.1 nTPM
pons: 4 nTPM
white matter: 3.9 nTPM
basal ganglia: 3.6 nTPM
medulla oblongata: 3.4 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about PHRF1.

Disease | ImmuneIEDB

Conditions an epitope on PHRF1 was assayed in.

skin melanoma T cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.38
gnomAD pLI: 0.06
gnomAD missense Z: -1.56
DepMap mean gene effect: 0.05
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

protein ubiquitination

Molecular functions

Cellular components

membrane

Protein domainsUniProt · Pfam · InterPro

Zinc finger, RING-type
Zinc finger, PHD-type
Zinc finger, FYVE/PHD-type
Zinc finger, RING/FYVE/PHD-type
Zinc finger, RING-type, conserved site
Zinc finger, PHD-finger
SFR19-like, C-terminal domain
PHD-finger
Ring finger domain
SCAF11-like, C-terminal domain
PHD and RING finger domain-containing protein 1/Atg35

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of PHRF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PHRF1 as an antibody target. Whether an autoantibody or antibody against PHRF1 could matter depends on whether native PHRF1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PHRF1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PHRF1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...