Seroatlas · Human Serome Atlas

PHF7

E3 ubiquitin-protein ligase PHF7

Also known as: HSPC226, NYD-SP6, PHF7_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9BWX1
Gene: PHF7
Ensembl: ENSG00000010318
Chromosome: 3
Canonical length: 381 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoplasm,Nuclear speckles,Golgi apparatus

OverviewNCBI Gene

Spermatogenesis is a complex process regulated by extracellular and intracellular factors as well as cellular interactions among interstitial cells of the testis, Sertoli cells, and germ cells. This gene is expressed in the testis in Sertoli cells but not germ cells. The protein encoded by this gene contains plant homeodomain (PHD) finger domains, also known as leukemia associated protein (LAP) domains, believed to be involved in transcriptional regulation. The protein, which localizes to the nucleus of transfected cells, has been implicated in the transcriptional regulation of spermatogenesis. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]

Canonical amino-acid sequenceUniProt

381 residues, UniProt reviewed canonical sequence.

>Q9BWX1|PHF7
     1  MKTVKEKKEC QRLRKSAKTR RVTQRKPSSG PVCWLCLREP GDPEKLGEFL QKDNISVHYF
    61  CLILSSKLPQ RGQSNRGFHG FLPEDIKKEA ARASRKICFV CKKKGAAINC QKDQCLRNFH
   121  LPCGQERGCL SQFFGEYKSF CDKHRPTQNI QHGHVGEESC ILCCEDLSQQ SVENIQSPCC
   181  SQAIYHRKCI QKYAHTSAKH FFKCPQCNNR KEFPQEMLRM GIHIPDRDAA WELEPGAFSD
   241  LYQRYQHCDA PICLYEQGRD SFEDEGRWCL ILCATCGSHG THRDCSSLRS NSKKWECEEC
   301  SPAAATDYIP ENSGDIPCCS STFHPEEHFC RDNTLEENPG LSWTDWPEPS LLEKPESSRG
   361  RRSYSWRSKG VRITNSCKKS K

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PHF7 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.44
Highest tissue expression: 1,149 nTPM

Expression across tissuesHPA

Tissue

testis: 1,149 nTPM
parathyroid gland: 5.3 nTPM
skin: 4.8 nTPM
breast: 4.7 nTPM
liver: 4.7 nTPM
adipose tissue: 4.6 nTPM

Single-cell type

late spermatids: 12,449 nCPM
late primary spermatocytes: 3,497 nCPM
early spermatids: 2,532 nCPM
early primary spermatocytes: 681 nCPM
sertoli cells: 62 nCPM
differentiating spermatogonia: 57 nCPM

Immune cell

memory B-cell: 3.4 nTPM
T-reg: 2.5 nTPM
eosinophil: 1.8 nTPM
naive B-cell: 1.8 nTPM
memory CD8 T-cell: 1.6 nTPM
naive CD8 T-cell: 1.6 nTPM

Brain region

cerebral cortex: 2.8 nTPM
cerebellum: 2.4 nTPM
pons: 2.4 nTPM
midbrain: 2.3 nTPM
white matter: 2.3 nTPM
medulla oblongata: 2.2 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.68
gnomAD pLI: 0
gnomAD missense Z: 1.14
DepMap mean gene effect: -0.06
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

negative regulation of osteoclast proliferation

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of PHF7 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PHF7 as an antibody target. Whether an autoantibody or antibody against PHF7 could matter depends on whether native PHF7 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PHF7 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PHF7 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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