Seroatlas · Human Serome Atlas

PHF20

PHD finger protein 20

Also known as: C20orf104, dJ1121G12.1, PHF20_HUMAN, TDRD20A

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q9BVI0
Gene
PHF20
Ensembl
ENSG00000025293
Chromosome
20
Canonical length
1012 aa
Protein class
Cancer-related genes, Plasma proteins, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Nuclear membrane
Quaternary structure
Homodimer

OverviewNCBI Gene

Predicted to enable DNA binding activity and zinc ion binding activity. Predicted to be involved in regulation of transcription by RNA polymerase II. Located in cytosol; nuclear membrane; and nucleoplasm. Part of MLL1 complex and NSL complex. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

1012 residues, UniProt reviewed canonical sequence.

>Q9BVI0|PHF20
     1  MTKHPPNRRG ISFEVGAQLE ARDRLKNWYP AHIEDIDYEE GKVLIHFKRW NHRYDEWFCW
    61  DSPYLRPLEK IQLRKEGLHE EDGSSEFQIN EQVLACWSDC RFYPAKVTAV NKDGTYTVKF
   121  YDGVVQTVKH IHVKAFSKDQ NIVGNARPKE TDHKSLSSSP DKREKFKEQR KATVNVKKDK
   181  EDKPLKTEKR PKQPDKEGKL ICSEKGKVSE KSLPKNEKED KENISENDRE YSGDAQVDKK
   241  PENDIVKSPQ ENLREPKRKR GRPPSIAPTA VDSNSQTLQP ITLELRRRKI SKGCEVPLKR
   301  PRLDKNSSQE KSKNYSENTD KDLSRRRSSR LSTNGTHEIL DPDLVVSDLV DTDPLQDTLS
   361  STKESEEGQL KSALEAGQVS SALTCHSFGD GSGAAGLELN CPSMGENTMK TEPTSPLVEL
   421  QEISTVEVTN TFKKTDDFGS SNAPAVDLDH KFRCKVVDCL KFFRKAKLLH YHMKYFHGME
   481  KSLEPEESPG KRHVQTRGPS ASDKPSQETL TRKRVSASSP TTKDKEKNKE KKFKEFVRVK
   541  PKKKKKKKKK TKPECPCSEE ISDTSQEPSP PKAFAVTRCG SSHKPGVHMS PQLHGPESGH
   601  HKGKVKALEE DNLSESSSES FLWSDDEYGQ DVDVTTNPDE ELDGDDRYDF EVVRCICEVQ
   661  EENDFMIQCE ECQCWQHGVC MGLLEENVPE KYTCYVCQDP PGQRPGFKYW YDKEWLSRGH
   721  MHGLAFLEEN YSHQNAKKIV ATHQLLGDVQ RVIEVLHGLQ LKMSILQSRE HPDLPLWCQP
   781  WKQHSGEGRS HFRNIPVTDT RSKEEAPSYR TLNGAVEKPR PLALPLPRSV EESYITSEHC
   841  YQKPRAYYPA VEQKLVVETR GSALDDAVNP LHENGDDSLS PRLGWPLDQD RSKGDSDPKP
   901  GSPKVKEYVS KKALPEEAPA RKLLDRGGEG LLSSQHQWQF NLLTHVESLQ DEVTHRMDSI
   961  EKELDVLESW LDYTGELEPP EPLARLPQLK HCIKQLLMDL GKVQQIALCC ST

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PHF20 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.59
Highest tissue expression
31 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 31 nTPM
  • tongue: 16 nTPM
  • blood vessel: 14 nTPM
  • spleen: 14 nTPM
  • thymus: 14 nTPM
  • retina: 13 nTPM

Single-cell type

  • neutrophils: 1,234 nCPM
  • mast cells: 1,109 nCPM
  • late spermatids: 1,082 nCPM
  • early spermatids: 504 nCPM
  • innate lymphoid cells: 482 nCPM
  • monocytes: 444 nCPM

Immune cell

  • eosinophil: 33 nTPM
  • neutrophil: 26 nTPM
  • basophil: 18 nTPM
  • non-classical monocyte: 17 nTPM
  • NK-cell: 16 nTPM
  • memory B-cell: 15 nTPM

Brain region

  • cerebellum: 38 nTPM
  • white matter: 35 nTPM
  • medulla oblongata: 31 nTPM
  • thalamus: 31 nTPM
  • basal ganglia: 30 nTPM
  • cerebral cortex: 30 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about PHF20.

Disease | GeneticClinVar

2 pathogenic / likely-pathogenic of 135 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.26
gnomAD pLI
1
gnomAD missense Z
2.35
DepMap mean gene effect
-0.12
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 12% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of PHF20 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PHF20 as an antibody target. Whether an autoantibody or antibody against PHF20 could matter depends on whether native PHF20 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PHF20 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PHF20 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/PHF20. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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