Seroatlas · Human Serome Atlas

PHACTR4

Phosphatase and actin regulator 4

Also known as: FLJ13171, PHAR4_HUMAN, PPP1R124

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8IZ21
Gene: PHACTR4
Ensembl: ENSG00000204138
Chromosome: 1
Canonical length: 702 aa
Protein class: Predicted intracellular proteins
Subcellular location: Plasma membrane,Intermediate filaments,Cytosol

OverviewNCBI Gene

This gene encodes a member of the phosphatase and actin regulator (PHACTR) family. Other PHACTR family members have been shown to inhibit protein phosphatase 1 (PP1) activity, and the homolog of this gene in the mouse has been shown to interact with actin and PP1. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

702 residues, UniProt reviewed canonical sequence.

>Q8IZ21|PHACTR4
     1  MEDPFEEADQ PTTEPGMVLD SVEAGDTTPP TKRKSKFSGF GKIFKPWKWR KKKSSDKFKE
    61  TSEVLERKIS MRKPREELVK RGVLLEDPEQ GGEDPGKPSD AMLKNGHTTP IGNARSSSPV
   121  QVEEEPVRLA SLRKAIPEED LKKRLGSTGS QPNSEAESVP ENVPKPPLLP PKRPLSSSHE
   181  ASEGQAKDAT SSGGTARFII STSITTAPAA TTAATSLAKT VNLSVTPSPA PRTLPAAPAS
   241  TNTTATPSLT HMVPAKQPPI PPPKPAHRNS NPVIAELSQA INSGTLLSKP SPPLPPKRGI
   301  PSTSVPTLES AAAITTKTPS DEREKSTCSM GSELLPMISP RSPSPPLPTH IPPEPPRTPP
   361  FPAKTFQVVP EIEFPPSLDL HQEIPQQEDQ KKEVPKRILD QNFGEPHIPS RLPPLPLHIR
   421  IQQALTSPLP MTPILEGSHR AHSLLFENSD SFSEDSSTLG RTRSLPITIE MLKVPDDEEE
   481  EEQTCPSTFS EEMTPTSVIP KLPQCLREEE EKESDSDSEG PIQYRDEEDE DESYQSALAN
   541  KVKRKDTLAM KLNHRPSEPE LNLNSWPCKS KEEWNEIRHQ IGNTLIRRLS QRPTPEELEQ
   601  RNILQPKNEA DRQAEKREIK RRLTRKLSQR PTVAELLARK ILRFNEYVEV TDAQDYDRRA
   661  DKPWTKLTPA DKAAIRKELN EFKSSEMEVH EESKHFTRYH RP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PHACTR4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.66
Highest tissue expression: 31 nTPM

Expression across tissuesHPA

Tissue

esophagus: 31 nTPM
tongue: 17 nTPM
liver: 15 nTPM
skin: 15 nTPM
parathyroid gland: 14 nTPM
vagina: 14 nTPM

Single-cell type

esophageal apical cells: 987 nCPM
podocytes: 753 nCPM
endometrial glandular cells: 734 nCPM
ocular epithelial cells: 496 nCPM
endometrial ciliated cells: 490 nCPM
megakaryocyte-erythroid progenitors: 473 nCPM

Immune cell

gdT-cell: 3.3 nTPM
memory CD8 T-cell: 3.3 nTPM
non-classical monocyte: 3.3 nTPM
MAIT T-cell: 2.9 nTPM
memory CD4 T-cell: 2.6 nTPM
naive CD8 T-cell: 2.6 nTPM

Brain region

choroid plexus: 28 nTPM
medulla oblongata: 26 nTPM
white matter: 25 nTPM
hypothalamus: 24 nTPM
thalamus: 24 nTPM
cerebellum: 24 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.69
gnomAD pLI: 0
gnomAD missense Z: 1.1
DepMap mean gene effect: -0.12
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of PHACTR4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

PPP1CA

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PHACTR4 as an antibody target. Whether an autoantibody or antibody against PHACTR4 could matter depends on whether native PHACTR4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PHACTR4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PHACTR4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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