PFKFB3

Protein identityUniProt · HPA

UniProt accession

Q16875

Gene

PFKFB3

Ensembl

ENSG00000170525

Chromosome

10

Canonical length

520 aa

Protein class

Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm

Quaternary structure

Homodimer

OverviewNCBI Gene

The protein encoded by this gene belongs to a family of bifunctional proteins that are involved in both the synthesis and degradation of fructose-2,6-bisphosphate, a regulatory molecule that controls glycolysis in eukaryotes. The encoded protein has a 6-phosphofructo-2-kinase activity that catalyzes the synthesis of fructose-2,6-bisphosphate (F2,6BP), and a fructose-2,6-biphosphatase activity that catalyzes the degradation of F2,6BP. This protein is required for cell cycle progression and prevention of apoptosis. It functions as a regulator of cyclin-dependent kinase 1, linking glucose metabolism to cell proliferation and survival in tumor cells. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2016]

Canonical amino-acid sequenceUniProt

520 residues, UniProt reviewed canonical sequence.

>Q16875|PFKFB3
     1  MPLELTQSRV QKIWVPVDHR PSLPRSCGPK LTNSPTVIVM VGLPARGKTY ISKKLTRYLN
    61  WIGVPTKVFN VGEYRREAVK QYSSYNFFRP DNEEAMKVRK QCALAALRDV KSYLAKEGGQ
   121  IAVFDATNTT RERRHMILHF AKENDFKAFF IESVCDDPTV VASNIMEVKI SSPDYKDCNS
   181  AEAMDDFMKR ISCYEASYQP LDPDKCDRDL SLIKVIDVGR RFLVNRVQDH IQSRIVYYLM
   241  NIHVQPRTIY LCRHGENEHN LQGRIGGDSG LSSRGKKFAS ALSKFVEEQN LKDLRVWTSQ
   301  LKSTIQTAEA LRLPYEQWKA LNEIDAGVCE ELTYEEIRDT YPEEYALREQ DKYYYRYPTG
   361  ESYQDLVQRL EPVIMELERQ ENVLVICHQA VLRCLLAYFL DKSAEEMPYL KCPLHTVLKL
   421  TPVAYGCRVE SIYLNVESVC THRERSEDAK KGPNPLMRRN SVTPLASPEP TKKPRINSFE
   481  EHVASTSAAL PSCLPPEVPT QLPGQNMKGS RSSADSSRKH

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PFKFB3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.3

Highest tissue expression

535 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.45

gnomAD pLI

0.25

gnomAD missense Z

1.66

DepMap mean gene effect

0.08

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• apoptotic process
• fructose 2,6-bisphosphate metabolic process
• fructose metabolic process
• glial cell differentiation
• gluconeogenesis
• glycolytic process
• motor behavior

Molecular functions

• 6-phosphofructo-2-kinase activity
• ATP binding
• fructose-2,6-bisphosphate 2-phosphatase activity

Cellular components

• 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase complex
• cytosol
• nucleoplasm

Protein domainsUniProt · Pfam · InterPro

• Phosphoglycerate/bisphosphoglycerate mutase, active site
• Fructose-2,6-bisphosphatase
• Histidine phosphatase superfamily, clade-1
• 6-phosphofructo-2-kinase
• P-loop containing nucleoside triphosphate hydrolase
• Histidine phosphatase superfamily
• Histidine phosphatase superfamily (branch 1)
• 6-phosphofructo-2-kinase

KeywordsUniProt

• ATP-binding
• Hydrolase
• Kinase
• Multifunctional enzyme
• Nucleotide-binding
• Phosphoprotein
• Transferase