PFKFB1

Protein identityUniProt · HPA

UniProt accession

P16118

Gene

PFKFB1

Ensembl

ENSG00000158571

Chromosome

X

Canonical length

471 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

Quaternary structure

Homodimer

OverviewNCBI Gene

This gene encodes a member of the family of bifunctional 6-phosphofructo-2-kinase:fructose-2,6-biphosphatase enzymes. The enzyme forms a homodimer that catalyzes both the synthesis and degradation of fructose-2,6-biphosphate using independent catalytic domains. Fructose-2,6-biphosphate is an activator of the glycolysis pathway and an inhibitor of the gluconeogenesis pathway. Consequently, regulating fructose-2,6-biphosphate levels through the activity of this enzyme is thought to regulate glucose homeostasis. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Nov 2012]

Canonical amino-acid sequenceUniProt

471 residues, UniProt reviewed canonical sequence.

>P16118|PFKFB1
     1  MSPEMGELTQ TRLQKIWIPH SSGSSRLQRR RGSSIPQFTN SPTMVIMVGL PARGKTYIST
    61  KLTRYLNWIG TPTKVFNLGQ YRREAVSYKN YEFFLPDNME ALQIRKQCAL AALKDVHNYL
   121  SHEEGHVAVF DATNTTRERR SLILQFAKEH GYKVFFIESI CNDPGIIAEN IRQVKLGSPD
   181  YIDCDREKVL EDFLKRIECY EVNYQPLDEE LDSHLSYIKI FDVGTRYMVN RVQDHIQSRT
   241  VYYLMNIHVT PRSIYLCRHG ESELNIRGRI GGDSGLSVRG KQYAYALANF IQSQGISSLK
   301  VWTSHMKRTI QTAEALGVPY EQWKALNEID AGVCEEMTYE EIQEHYPEEF ALRDQDKYRY
   361  RYPKGESYED LVQRLEPVIM ELERQENVLV ICHQAVMRCL LAYFLDKSSD ELPYLKCPLH
   421  TVLKLTPVAY GCKVESIYLN VEAVNTHREK PENVDITREP EEALDTVPAH Y

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PFKFB1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.26

Highest tissue expression

105 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.89

gnomAD pLI

0

gnomAD missense Z

-0.05

DepMap mean gene effect

-0.05

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• fructose 2,6-bisphosphate metabolic process
• fructose metabolic process
• gluconeogenesis
• glycolytic process
• negative regulation of glycolytic process through fructose-6-phosphate

Molecular functions

• 6-phosphofructo-2-kinase activity
• ATP binding
• fructose-2,6-bisphosphate 2-phosphatase activity
• identical protein binding

Cellular components

• 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase complex
• cytosol

Protein domainsUniProt · Pfam · InterPro

• Phosphoglycerate/bisphosphoglycerate mutase, active site
• Fructose-2,6-bisphosphatase
• Histidine phosphatase superfamily, clade-1
• 6-phosphofructo-2-kinase
• P-loop containing nucleoside triphosphate hydrolase
• Histidine phosphatase superfamily
• Histidine phosphatase superfamily (branch 1)
• 6-phosphofructo-2-kinase

KeywordsUniProt

• Acetylation
• ATP-binding
• Hydrolase
• Kinase
• Multifunctional enzyme
• Nucleotide-binding
• Phosphoprotein
• Transferase

InteractionsUniProt · HPA

Protein binding partners of PFKFB1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• PFKFB4