Seroatlas · Human Serome Atlas

PDIA5

Protein disulfide-isomerase A5

Also known as: FLJ30401, PDIA5_HUMAN, PDIR

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q14554
Gene
PDIA5
Ensembl
ENSG00000065485
Chromosome
3
Canonical length
519 aa
Protein class
Enzymes, Metabolic proteins, Predicted intracellular proteins
Subcellular location
Endoplasmic reticulum
Secretome location
Intracellular and membrane

OverviewNCBI Gene

This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal ER-signal sequence, three catalytically active thioredoxin (TRX) domains, a TRX-like domain, and a C-terminal ER-retention sequence. The N-terminal TRX-like domain is the primary binding site for the major ER chaperone calreticulin and possibly other proteins and substrates as well. Alternative splicing results in multiple protein- and non-protein-coding transcript variants. [provided by RefSeq, Dec 2016]

Canonical amino-acid sequenceUniProt

519 residues, UniProt reviewed canonical sequence.

>Q14554|PDIA5
     1  MARAGPAWLL LAIWVVLPSW LSSAKVSSLI ERISDPKDLK KLLRTRNNVL VLYSKSEVAA
    61  ENHLRLLSTV AQAVKGQGTI CWVDCGDAES RKLCKKMKVD LSPKDKKVEL FHYQDGAFHT
   121  EYNRAVTFKS IVAFLKDPKG PPLWEEDPGA KDVVHLDSEK DFRRLLKKEE KPLLIMFYAP
   181  WCSMCKRMMP HFQKAATQLR GHAVLAGMNV YSSEFENIKE EYSVRGFPTI CYFEKGRFLF
   241  QYDNYGSTAE DIVEWLKNPQ PPQPQVPETP WADEGGSVYH LTDEDFDQFV KEHSSVLVMF
   301  HAPWCGHCKK MKPEFEKAAE ALHGEADSSG VLAAVDATVN KALAERFHIS EFPTLKYFKN
   361  GEKYAVPVLR TKKKFLEWMQ NPEAPPPPEP TWEEQQTSVL HLVGDNFRET LKKKKHTLVM
   421  FYAPWCPHCK KVIPHFTATA DAFKDDRKIA CAAVDCVKDK NQDLCQQEAV KGYPTFHYYH
   481  YGKFAEKYDS DRTELGFTNY IRALREGDHE RLGKKKEEL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PDIA5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.31
Highest tissue expression
75 nTPM

Expression across tissuesHPA

Tissue

  • liver: 75 nTPM
  • placenta: 45 nTPM
  • stomach: 32 nTPM
  • prostate: 30 nTPM
  • thyroid gland: 29 nTPM
  • epididymis: 29 nTPM

Single-cell type

  • extravillous trophoblasts: 250 nCPM
  • megakaryocyte progenitors: 107 nCPM
  • hepatocytes: 104 nCPM
  • migrating cytotrophoblasts: 99 nCPM
  • rod photoreceptor cells: 96 nCPM
  • plasma cells: 86 nCPM

Immune cell

  • plasmacytoid DC: 13 nTPM
  • classical monocyte: 9.3 nTPM
  • myeloid DC: 5.5 nTPM
  • memory B-cell: 3.5 nTPM
  • intermediate monocyte: 2.4 nTPM
  • MAIT T-cell: 2.3 nTPM

Brain region

  • choroid plexus: 6.9 nTPM
  • cerebral cortex: 4.2 nTPM
  • thalamus: 3.4 nTPM
  • hypothalamus: 3.2 nTPM
  • white matter: 2.9 nTPM
  • midbrain: 2.8 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.84
gnomAD pLI
0
gnomAD missense Z
0.24
DepMap mean gene effect
0.16
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of PDIA5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads PDIA5 as an antibody target. Whether an autoantibody or antibody against PDIA5 could matter depends on whether native PDIA5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

PDIA5 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label PDIA5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/PDIA5. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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