PDE1B

Protein identityUniProt · HPA

UniProt accession

Q01064

Gene

PDE1B

Ensembl

ENSG00000123360

Chromosome

12

Canonical length

536 aa

Protein class

Enzymes, FDA approved drug targets, Metabolic proteins, Plasma proteins, Predicted intracellular proteins

Quaternary structure

Homodimer

OverviewNCBI Gene

The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE1 subfamily. Members of the PDE1 family are calmodulin-dependent PDEs that are stimulated by a calcium-calmodulin complex. This PDE has dual-specificity for the second messengers, cAMP and cGMP, with a preference for cGMP as a substrate. cAMP and cGMP function as key regulators of many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]

Canonical amino-acid sequenceUniProt

536 residues, UniProt reviewed canonical sequence.

>Q01064|PDE1B
     1  MELSPRSPPE MLEESDCPSP LELKSAPSKK MWIKLRSLLR YMVKQLENGE INIEELKKNL
    61  EYTASLLEAV YIDETRQILD TEDELQELRS DAVPSEVRDW LASTFTQQAR AKGRRAEEKP
   121  KFRSIVHAVQ AGIFVERMFR RTYTSVGPTY STAVLNCLKN LDLWCFDVFS LNQAADDHAL
   181  RTIVFELLTR HNLISRFKIP TVFLMSFLDA LETGYGKYKN PYHNQIHAAD VTQTVHCFLL
   241  RTGMVHCLSE IELLAIIFAA AIHDYEHTGT TNSFHIQTKS ECAIVYNDRS VLENHHISSV
   301  FRLMQDDEMN IFINLTKDEF VELRALVIEM VLATDMSCHF QQVKTMKTAL QQLERIDKPK
   361  ALSLLLHAAD ISHPTKQWLV HSRWTKALME EFFRQGDKEA ELGLPFSPLC DRTSTLVAQS
   421  QIGFIDFIVE PTFSVLTDVA EKSVQPLADE DSKSKNQPSF QWRQPSLDVE VGDPNPDVVS
   481  FRSTWVKRIQ ENKQKWKERA ASGITNQMSI DELSPCEEEA PPSPAEDEHN QNGNLD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PDE1B can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.35

Highest tissue expression

96 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.43

gnomAD pLI

0.34

gnomAD missense Z

2.27

DepMap mean gene effect

-0.04

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cellular response to granulocyte macrophage colony-stimulating factor stimulus
• cellular response to macrophage colony-stimulating factor stimulus
• dopamine catabolic process
• locomotory behavior
• monocyte differentiation
• negative regulation of cAMP/PKA signal transduction
• response to amphetamine
• serotonin metabolic process
• signal transduction
• visual learning

Molecular functions

• 3',5'-cyclic-AMP phosphodiesterase activity
• 3',5'-cyclic-GMP phosphodiesterase activity
• calmodulin binding
• calmodulin-activated 3',5'-cyclic-GMP phosphodiesterase activity
• calmodulin-activated dual specificity 3',5'-cyclic-GMP, 3',5'-cyclic-AMP phosphodiesterase activity
• metal ion binding

Cellular components

• cytosol
• neuronal cell body

Protein domainsUniProt · Pfam · InterPro

• 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain
• HD/PDEase domain
• PDE1, N-terminal domain
• 3'5'-cyclic nucleotide phosphodiesterase
• 3'5'-cyclic nucleotide phosphodiesterase, conserved site
• 3'5'-cyclic nucleotide phosphodiesterase, catalytic domain superfamily
• 3'5'-cyclic nucleotide phosphodiesterase
• 3'5'-cyclic nucleotide phosphodiesterase N-terminal

KeywordsUniProt

• Calmodulin-binding
• cAMP
• cGMP
• Cytoplasm
• Hydrolase
• Metal-binding
• Phosphoprotein
• Zinc