PAPOLG

Protein identityUniProt · HPA

UniProt accession

Q9BWT3

Gene

PAPOLG

Ensembl

ENSG00000115421

Chromosome

2

Canonical length

736 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins, Predicted membrane proteins

Subcellular location

Nucleoplasm,Nuclear bodies,Cytosol

OverviewNCBI Gene

This gene encodes a member of the poly(A) polymerase family which catalyzes template-independent extension of the 3' end of a DNA/RNA strand. This enzyme shares 60% identity to the well characterized poly(A) polymerase II (PAPII) at the amino acid level. These two enzymes have similar organization of structural and functional domains. This enzyme is exclusively localized in the nucleus and exhibits both nonspecific and CPSF (cleavage and polyadenylation specificity factor)/AAUAAA-dependent polyadenylation activity. This gene is located on chromosome 2 in contrast to the PAPII gene, which is located on chromosome 14. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

736 residues, UniProt reviewed canonical sequence.

>Q9BWT3|PAPOLG
     1  MKEMSANTVL DSQRQQKHYG ITSPISLASP KEIDHIYTQK LIDAMKPFGV FEDEEELNHR
    61  LVVLGKLNNL VKEWISDVSE SKNLPPSVVA TVGGKIFTFG SYRLGVHTKG ADIDALCVAP
   121  RHVERSDFFQ SFFEKLKHQD GIRNLRAVED AFVPVIKFEF DGIEIDLVFA RLAIQTISDN
   181  LDLRDDSRLR SLDIRCIRSL NGCRVTDEIL HLVPNKETFR LTLRAVKLWA KRRGIYSNML
   241  GFLGGVSWAM LVARTCQLYP NAAASTLVHK FFLVFSKWEW PNPVLLKQPE ESNLNLPVWD
   301  PRVNPSDRYH LMPIITPAYP QQNSTYNVST STRTVMVEEF KQGLAVTDEI LQGKSDWSKL
   361  LEPPNFFQKY RHYIVLTASA STEENHLEWV GLVESKIRVL VGNLERNEFI TLAHVNPQSF
   421  PGNKEHHKDN NYVSMWFLGI IFRRVENAES VNIDLTYDIQ SFTDTVYRQA NNINMLKEGM
   481  KIEATHVKKK QLHHYLPAEI LQKKKKQSLS DVNRSSGGLQ SKRLSLDSSC LDSSRDTDNG
   541  TPFNSPASKS DSPSVGETER NSAEPAAVIV EKPLSVPPAQ GLSIPVIGAK VDSTVKTVSP
   601  PTVCTIPTVV GRNVIPRITT PHNPAQGQPH LNGMSNITKT VTPKRSHSPS IDGTPKRLKD
   661  VEKFIRLEST FKDPRTAEER KRKSVDAIGG ESMPIPTIDT SRKKRLPSKE LPDSSSPVPA
   721  NNIRVIKNSI RLTLNR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against PAPOLG can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.4

Highest tissue expression

4 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.27

gnomAD pLI

1

gnomAD missense Z

2.2

DepMap mean gene effect

0.03

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• mRNA processing
• RNA 3'-end processing

Molecular functions

• ATP binding
• metal ion binding
• poly(A) RNA polymerase activity
• RNA binding

Cellular components

• cytosol
• membrane
• nuclear body
• nucleoplasm
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Poly(A) polymerase, RNA-binding domain
• Poly(A) polymerase, central domain
• Nucleotidyltransferase, class I-like, C-terminal
• Nucleotidyltransferase superfamily
• Poly(A) polymerase, nucleotidyltransferase domain
• Poly(A) polymerase predicted RNA binding domain
• Poly(A) polymerase central domain
• Poly(A) polymerase nucleotidyltransferase domain

KeywordsUniProt

• Acetylation
• ATP-binding
• Magnesium
• Manganese
• Metal-binding
• mRNA processing
• Nucleotide-binding
• Nucleus
• Phosphoprotein
• RNA-binding
• Transferase