Seroatlas · Human Serome Atlas

OTUD3

OTU domain-containing protein 3

Also known as: DUBA4, KIAA0459, OTUD3_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q5T2D3
Gene: OTUD3
Ensembl: ENSG00000169914
Chromosome: 1
Canonical length: 398 aa
Protein class: Enzymes, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Intermediate filaments,Cytosol

OverviewNCBI Gene

Enables cysteine-type deubiquitinase activity. Involved in cellular response to nutrient levels and protein deubiquitination. Acts upstream of or within negative regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction; protein deubiquitination; and protein stabilization. Located in cytoplasm. Is active in nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

398 residues, UniProt reviewed canonical sequence.

>Q5T2D3|OTUD3
     1  MSRKQAAKSR PGSGSRKAEA ERKRDERAAR RALAKERRNR PESGGGGGCE EEFVSFANQL
    61  QALGLKLREV PGDGNCLFRA LGDQLEGHSR NHLKHRQETV DYMIKQREDF EPFVEDDIPF
   121  EKHVASLAKP GTFAGNDAIV AFARNHQLNV VIHQLNAPLW QIRGTEKSSV RELHIAYRYG
   181  EHYDSVRRIN DNSEAPAHLQ TDFQMLHQDE SNKREKIKTK GMDSEDDLRD EVEDAVQKVC
   241  NATGCSDFNL IVQNLEAENY NIESAIIAVL RMNQGKRNNA EENLEPSGRV LKQCGPLWEE
   301  GGSGARIFGN QGLNEGRTEN NKAQASPSEE NKANKNQLAK VTNKQRREQQ WMEKKKRQEE
   361  RHRHKALESR GSHRDNNRSE AEANTQVTLV KTFAALNI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against OTUD3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.43
Highest tissue expression: 10 nTPM

Expression across tissuesHPA

Tissue

testis: 10 nTPM
cerebellum: 10 nTPM
retina: 8.6 nTPM
endometrium: 6.9 nTPM
ovary: 6.3 nTPM
cervix: 6.1 nTPM

Single-cell type

tuft cells: 46 nCPM
sertoli cells: 41 nCPM
gastric progenitor cells: 41 nCPM
brain inhibitory neurons: 40 nCPM
retinal amacrine cells: 39 nCPM
differentiating spermatogonia: 36 nCPM

Immune cell

memory B-cell: 0.3 nTPM
memory CD4 T-cell: 0.3 nTPM
naive CD4 T-cell: 0.3 nTPM
naive CD8 T-cell: 0.3 nTPM
gdT-cell: 0.2 nTPM
MAIT T-cell: 0.2 nTPM

Brain region

cerebral cortex: 15 nTPM
cerebellum: 13 nTPM
white matter: 13 nTPM
basal ganglia: 13 nTPM
hippocampal formation: 12 nTPM
amygdala: 11 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.92
gnomAD pLI: 0
gnomAD missense Z: 0.77
DepMap mean gene effect: -0.07
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

cysteine-type deubiquitinase activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads OTUD3 as an antibody target. Whether an autoantibody or antibody against OTUD3 could matter depends on whether native OTUD3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

OTUD3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label OTUD3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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