Seroatlas · Human Serome Atlas

NWD1

NACHT domain- and WD repeat-containing protein 1

Also known as: NWD1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q149M9
Gene: NWD1
Ensembl: ENSG00000188039
Chromosome: 19
Canonical length: 1564 aa
Protein class: Predicted intracellular proteins
Subcellular location: Nucleoli,Cytosol

OverviewNCBI Gene

The protein encoded by this gene is thought to be a cytosolic protein and predicted to contain a NACHT domain and multiple WD40 repeats. Increased expression of this gene was observed in some prostate cancer cell lines. Knocking down expression of this gene results in decreased androgen receptor protein levels, indicating that this gene may be important in modulating androgen receptor activity. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2016]

Canonical amino-acid sequenceUniProt

1564 residues, UniProt reviewed canonical sequence.

>Q149M9|NWD1
     1  MQRGKPCRAL PTLKCQTFCQ RHGLMFEVVD LRWGIRNIEA TDHLTTELCL EEVDRCWKTS
    61  IGPAFVALIG DQYGPCLIPS RIDEKEWEVL RDHLTARPSD LELVARYFQR DENAFPPTYV
   121  LQAPGTGEAC EPEEATLTSV LRSGAQEARR LGLITQEQWQ HYHRSVIEWE IERSLLSSED
   181  REQGATVFLR EIQDLHKHIL EDCALRMVDR LADGCLDADA QNLLSSLKSH ITDMHPGVLK
   241  THRLPWSRDL VNPKNKTHAC YLKELGEQFV VRANHQVLTR LRELDTAGQE LAWLYQEIRH
   301  HLWQSSEVIQ TFCGRQELLA RLGQQLRHDD SKQHTPLVLF GPPGIGKTAL MCKLAEQMPR
   361  LLGHKTVTVL RLLGTSQMSS DARGLLKSIC FQVCLAYGLP LPPAQVLDAH TRVVQFFHTL
   421  LHTVSCRNFE SLVLLLDAMD DLDSVRHARR VPWLPLNCPP RVHLILSACS GALGVLDTLQ
   481  RVLLDPEAYW EVKPLSGNQG QQMIQLLLAA ARRTLSPVHT DLLWASLPEC GNPGRLRLAF
   541  EEARKWASFT VPVPLATTAE EATHQLCTRL EQTHGQLLVA HVLGYIVSSR HGLSEAELKD
   601  VLSLDDEVLQ DVYRDWTPPS KELLRFPPLL WVRLRRDLGY YLARRPVDGF TLLAIAHRQL
   661  VEVVRERYLS GSERAKRHGV LADFFSGTWS QGTKKLITLP LVGKPLNLDR KVAPQPLWFS
   721  HTVANLRKLK ELPYHLLHSG RLEELKQEVL GSMSWISCRG ISGGIEDLLD DFDLCAPHLD
   781  SPEVGLVREA LQLCRPAVEL RGMERSLLYT ELLARLHFFA TSHPALVGQL CQQAQSWFQL
   841  CAHPVLVPLG GFLQPPGGPL RATLSGCHKG ITAMAWGVEE KLLVIGTQDG IMAVWDMEEQ
   901  HVIHMLTGHT GEVRCVKIFA KGTLANSASK DYTLHLWNLL SGQEKFTIWD GGSKNPAEPQ
   961  IWNLHVDEAH KVVYSASGSK INAWNLETAE PVFHILGDAS DPWMCMAVLA SQATLLTVSR
  1021  DGVVSLWSSA TGKLQGKQHM SSIKEETPTC AVSVQKQGKL VTGFSNGSIS LVSSKGDRLL
  1081  EKLPDAVRFL VVSEDESLLA AGFGRSVRIF LADSRGFRRF MAMDLEHEDM VETAVFGTEN
  1141  NLIITGSLDA LIQVWSLSEQ GTLLDILEGV GAPVSLLARG GALVASASPQ SSSFKVWDLS
  1201  DAHRSRVPAP FLDRTGLTAV SHNGSYVYFP KIGDKNKVTI WDLAEGEEQD SLDTSSEIRC
  1261  LEVAEQRKLL FTGLVSGVVL VFPLNSRQDV ICIPPPEARK AINCMSLSKC EDRLAIAYDN
  1321  IVLVLDITSG DPCPVIDGPR YTFYTQLPET LSSVAILTDY RVVYSMTNGD LFLYECATSK
  1381  AFPLETHRSR VACVEVSHKE QLVVSGSEDA LLCLWDLQAR KWKFEMSYTS SYCRGVQCAC
  1441  FSKDDKYVYV GLKDRSILVW SVLDGTLLTV QFVHAVVNRI IPTTSGFIAP TRHGYLIREN
  1501  FQCLSAKASP QDPLKNFKKA MWMVKSRQRE ELVAAAGAPQ DLESESAQGN ETKSNKCSQV
  1561  CLIV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NWD1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.24
Highest tissue expression: 77 nTPM

Expression across tissuesHPA

Tissue

choroid plexus: 77 nTPM
fallopian tube: 12 nTPM
prostate: 6.4 nTPM
basal ganglia: 5.9 nTPM
cerebral cortex: 5.8 nTPM
amygdala: 4.7 nTPM

Single-cell type

choroid plexus epithelial cells: 2,510 nCPM
respiratory ciliated cells: 324 nCPM
endometrial ciliated cells: 293 nCPM
epididymal efferent duct ciliated cells: 260 nCPM
ependymal cells: 215 nCPM
fallopian tube ciliated cells: 165 nCPM

Immune cell

intermediate monocyte: 0.6 nTPM
basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

choroid plexus: 389 nTPM
hippocampal formation: 24 nTPM
thalamus: 21 nTPM
midbrain: 18 nTPM
cerebellum: 16 nTPM
basal ganglia: 13 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.99
gnomAD pLI: 0
gnomAD missense Z: 0.16
DepMap mean gene effect: -0.24
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

ATP binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NWD1 as an antibody target. Whether an autoantibody or antibody against NWD1 could matter depends on whether native NWD1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NWD1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NWD1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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