Seroatlas · Human Serome Atlas

NT5E

5'-nucleotidase

Also known as: 5NTD_HUMAN, CALJA, CD73, eN, eNT, NT5

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P21589
Gene: NT5E
Ensembl: ENSG00000135318
Chromosome: 6
Canonical length: 574 aa
Protein class: CD markers, Disease related genes, Enzymes, Human disease related genes, Metabolic proteins, Potential drug targets, Predicted intracellular proteins, Predicted membrane proteins
Subcellular location: Nucleoplasm,Plasma membrane,Cytosol
Secretome location: Intracellular and membrane
Quaternary structure: Homodimer

OverviewNCBI Gene

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

Canonical amino-acid sequenceUniProt

574 residues, UniProt reviewed canonical sequence.

>P21589|NT5E
     1  MCPRAARAPA TLLLALGAVL WPAAGAWELT ILHTNDVHSR LEQTSEDSSK CVNASRCMGG
    61  VARLFTKVQQ IRRAEPNVLL LDAGDQYQGT IWFTVYKGAE VAHFMNALRY DAMALGNHEF
   121  DNGVEGLIEP LLKEAKFPIL SANIKAKGPL ASQISGLYLP YKVLPVGDEV VGIVGYTSKE
   181  TPFLSNPGTN LVFEDEITAL QPEVDKLKTL NVNKIIALGH SGFEMDKLIA QKVRGVDVVV
   241  GGHSNTFLYT GNPPSKEVPA GKYPFIVTSD DGRKVPVVQA YAFGKYLGYL KIEFDERGNV
   301  ISSHGNPILL NSSIPEDPSI KADINKWRIK LDNYSTQELG KTIVYLDGSS QSCRFRECNM
   361  GNLICDAMIN NNLRHTDEMF WNHVSMCILN GGGIRSPIDE RNNGTITWEN LAAVLPFGGT
   421  FDLVQLKGST LKKAFEHSVH RYGQSTGEFL QVGGIHVVYD LSRKPGDRVV KLDVLCTKCR
   481  VPSYDPLKMD EVYKVILPNF LANGGDGFQM IKDELLRHDS GDQDINVVST YISKMKVIYP
   541  AVEGRIKFST GSHCHGSFSL IFLSLWAVIF VLYQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NT5E can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Cell surface
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.24
Highest tissue expression: 106 nTPM

Expression across tissuesHPA

Tissue

cervix: 106 nTPM
retina: 87 nTPM
smooth muscle: 69 nTPM
liver: 51 nTPM
endometrium: 46 nTPM
duodenum: 38 nTPM

Single-cell type

rod photoreceptor cells: 284 nCPM
corticotrophs: 176 nCPM
pituicytes/fscs: 118 nCPM
endometrial glandular cells: 116 nCPM
oligodendrocyte progenitor cells: 89 nCPM
respiratory deuterosomal cells: 80 nCPM

Immune cell

naive B-cell: 33 nTPM
naive CD8 T-cell: 31 nTPM
memory B-cell: 24 nTPM
memory CD8 T-cell: 12 nTPM
MAIT T-cell: 6.5 nTPM
total PBMC: 6.4 nTPM

Brain region

choroid plexus: 27 nTPM
spinal cord: 27 nTPM
midbrain: 25 nTPM
pons: 23 nTPM
thalamus: 23 nTPM
white matter: 21 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about NT5E.

Disease | AllUniProt

Conditions NT5E is implicated in, by any mechanism.

Calcification of joints and arteries (CALJA) MIM:211800

Disease | GeneticClinVar

4 pathogenic / likely-pathogenic of 119 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Hereditary arterial and articular multiple calcification syndrome
NT5E-related disorder

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.18
gnomAD pLI: 0
gnomAD missense Z: 0.6
DepMap mean gene effect: -0.06
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Calcineurin-like, phosphoesterase domain
Metallo-dependent phosphatase-like
Calcineurin-like phosphoesterase
5'-Nucleotidase, conserved site
5'-Nucleotidase/apyrase
5'-Nucleotidase, C-terminal
5'-Nucleotidase, C-terminal domain superfamily
5'-nucleotidase, C-terminal domain

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NT5E as an antibody target. Whether an autoantibody or antibody against NT5E could matter depends on whether native NT5E is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NT5E is annotated at the cell surface, where native NT5E is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label NT5E as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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