Seroatlas · Human Serome Atlas

NSUN5

28S rRNA (cytosine-C(5))-methyltransferase

Also known as: FLJ10267, NOL1R, NSUN5_HUMAN, NSUN5A, p120(NOL1), WBSCR20, WBSCR20A, Ynl022cL

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q96P11
Gene
NSUN5
Ensembl
ENSG00000130305
Chromosome
7
Canonical length
429 aa
Protein class
Disease related genes, Human disease related genes, Predicted intracellular proteins
Subcellular location
Nucleoplasm,Nucleoli

OverviewNCBI Gene

This gene encodes a member of an evolutionarily conserved family of proteins that may function as methyltransferases. This gene is located in a larger region of chromosome 7 that is deleted in Williams-Beuren syndrome, a multisystem developmental disorder. There are two pseudogenes for this gene located in the same region of chromosome 7. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Canonical amino-acid sequenceUniProt

429 residues, UniProt reviewed canonical sequence.

>Q96P11|NSUN5
     1  MGLYAAAAGV LAGVESRQGS IKGLVYSSNF QNVKQLYALV CETQRYSAVL DAVIASAGLL
    61  RAEKKLRPHL AKVLVYELLL GKGFRGGGGR WKALLGRHQA RLKAELARLK VHRGVSRNED
   121  LLEVGSRPGP ASQLPRFVRV NTLKTCSDDV VDYFKRQGFS YQGRASSLDD LRALKGKHFL
   181  LDPLMPELLV FPAQTDLHEH PLYRAGHLIL QDRASCLPAM LLDPPPGSHV IDACAAPGNK
   241  TSHLAALLKN QGKIFAFDLD AKRLASMATL LARAGVSCCE LAEEDFLAVS PSDPRYHEVH
   301  YILLDPSCSG SGMPSRQLEE PGAGTPSPVR LHALAGFQQR ALCHALTFPS LQRLVYSTCS
   361  LCQEENEDVV RDALQQNPGA FRLAPALPAW PHRGLSTFPG AEHCLRASPE TTLSSGFFVA
   421  VIERVEVPR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NSUN5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.24
Highest tissue expression
33 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 33 nTPM
  • bone marrow: 31 nTPM
  • spleen: 21 nTPM
  • pancreas: 20 nTPM
  • testis: 17 nTPM
  • cerebellum: 17 nTPM

Single-cell type

  • oocytes: 138 nCPM
  • neutrophil progenitors: 110 nCPM
  • extravillous trophoblasts: 60 nCPM
  • hofbauer cells: 47 nCPM
  • differentiating spermatogonia: 47 nCPM
  • early primary spermatocytes: 46 nCPM

Immune cell

  • naive B-cell: 47 nTPM
  • memory B-cell: 33 nTPM
  • plasmacytoid DC: 32 nTPM
  • naive CD4 T-cell: 32 nTPM
  • neutrophil: 28 nTPM
  • T-reg: 27 nTPM

Brain region

  • white matter: 14 nTPM
  • medulla oblongata: 14 nTPM
  • pons: 12 nTPM
  • cerebellum: 11 nTPM
  • spinal cord: 11 nTPM
  • cerebral cortex: 11 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
1.3
gnomAD pLI
0
gnomAD missense Z
0.17
DepMap mean gene effect
-0.2
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NSUN5 as an antibody target. Whether an autoantibody or antibody against NSUN5 could matter depends on whether native NSUN5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NSUN5 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NSUN5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/NSUN5. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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