NRF1

Protein identityUniProt · HPA

UniProt accession

Q16656

Gene

NRF1

Ensembl

ENSG00000106459

Chromosome

7

Canonical length

503 aa

Protein class

Metabolic proteins, Predicted intracellular proteins, Transcription factors

Subcellular location

Nucleoplasm,Cytosol

Quaternary structure

Homodimer

OverviewNCBI Gene

This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternative splicing results in multiple transcript variants. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for """"""""""""""""""""""""""""""""nuclear factor (erythroid-derived 2)-like 1"""""""""""""""""""""""""""""""" which has an official symbol of NFE2L1. [provided by RefSeq, May 2014]

Canonical amino-acid sequenceUniProt

503 residues, UniProt reviewed canonical sequence.

>Q16656|NRF1
     1  MEEHGVTQTE HMATIEAHAV AQQVQQVHVA TYTEHSMLSA DEDSPSSPED TSYDDSDILN
    61  STAADEVTAH LAAAGPVGMA AAAAVATGKK RKRPHVFESN PSIRKRQQTR LLRKLRATLD
   121  EYTTRVGQQA IVLCISPSKP NPVFKVFGAA PLENVVRKYK SMILEDLESA LAEHAPAPQE
   181  VNSELPPLTI DGIPVSVDKM TQAQLRAFIP EMLKYSTGRG KPGWGKESCK PIWWPEDIPW
   241  ANVRSDVRTE EQKQRVSWTQ ALRTIVKNCY KQHGREDLLY AFEDQQTQTQ ATATHSIAHL
   301  VPSQTVVQTF SNPDGTVSLI QVGTGATVAT LADASELPTT VTVAQVNYSA VADGEVEQNW
   361  ATLQGGEMTI QTTQASEATQ AVASLAEAAV AASQEMQQGA TVTMALNSEA AAHAVATLAE
   421  ATLQGGGQIV LSGETAAAVG ALTGVQDANG LVQIPVSMYQ TVVTSLAQGN GPVQVAMAPV
   481  TTRISDSAVT MDGQAVEVVT LEQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NRF1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.48

Highest tissue expression

17 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.19

gnomAD pLI

1

gnomAD missense Z

4.51

DepMap mean gene effect

-1.24

DepMap dependency class

common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• positive regulation of transcription by RNA polymerase II
• regulation of transcription by RNA polymerase II

Molecular functions

• DNA-binding transcription activator activity, RNA polymerase II-specific
• DNA-binding transcription factor activity, RNA polymerase II-specific
• protein homodimerization activity
• RNA polymerase II cis-regulatory region sequence-specific DNA binding

Cellular components

• cytosol
• extracellular exosome
• nucleoplasm
• nucleus

Protein domainsUniProt · Pfam · InterPro

• Nuclear respiratory factor 1, NLS/DNA-binding, dimerisation domain
• NRF1/Ewg family
• NLS-binding and DNA-binding and dimerisation domains of Nrf1

KeywordsUniProt

• Activator
• DNA-binding
• Isopeptide bond
• Nucleus
• Phosphoprotein
• Transcription
• Transcription regulation
• Ubl conjugation

InteractionsUniProt · HPA

Protein binding partners of NRF1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• PPRC1
• DYNLL1
• FHL2