Seroatlas · Human Serome Atlas

NOSIP

Nitric oxide synthase-interacting protein

Also known as: CGI-25, NOSIP_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9Y314
Gene: NOSIP
Ensembl: ENSG00000142546
Chromosome: 19
Canonical length: 301 aa
Protein class: Enzymes, Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

The protein encoded by this gene may modulate the activity and localization of nitric oxide synthase (endothelial and neuronal) and thus nitric oxide production. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2012]

Canonical amino-acid sequenceUniProt

301 residues, UniProt reviewed canonical sequence.

>Q9Y314|NOSIP
     1  MTRHGKNCTA GAVYTYHEKK KDTAASGYGT QNIRLSRDAV KDFDCCCLSL QPCHDPVVTP
    61  DGYLYEREAI LEYILHQKKE IARQMKAYEK QRGTRREEQK ELQRAASQDH VRGFLEKESA
   121  IVSRPLNPFT AKALSGTSPD DVQPGPSVGP PSKDKDKVLP SFWIPSLTPE AKATKLEKPS
   181  RTVTCPMSGK PLRMSDLTPV HFTPLDSSVD RVGLITRSER YVCAVTRDSL SNATPCAVLR
   241  PSGAVVTLEC VEKLIRKDMV DPVTGDKLTD RDIIVLQRGG TGFAGSGVKL QAEKSRPVMQ
   301  A

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NOSIP can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.42
Highest tissue expression: 136 nTPM

Expression across tissuesHPA

Tissue

testis: 136 nTPM
skeletal muscle: 126 nTPM
pancreas: 111 nTPM
tongue: 61 nTPM
spleen: 59 nTPM
heart muscle: 54 nTPM

Single-cell type

late spermatids: 1,226 nCPM
late primary spermatocytes: 605 nCPM
early spermatids: 346 nCPM
pancreatic acinar cells: 295 nCPM
esophageal apical cells: 289 nCPM
oocytes: 193 nCPM

Immune cell

naive CD4 T-cell: 844 nTPM
total PBMC: 580 nTPM
memory CD4 T-cell: 521 nTPM
naive CD8 T-cell: 468 nTPM
MAIT T-cell: 330 nTPM
T-reg: 305 nTPM

Brain region

white matter: 41 nTPM
cerebellum: 41 nTPM
pons: 40 nTPM
basal ganglia: 39 nTPM
thalamus: 39 nTPM
medulla oblongata: 39 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.06
gnomAD pLI: 0
gnomAD missense Z: 2.11
DepMap mean gene effect: -0.21
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Zinc finger, RING/FYVE/PHD-type
Nitric oxide synthase-interacting protein
Nitric oxide synthase-interacting protein, zinc-finger
Zinc-finger of nitric oxide synthase-interacting protein

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NOSIP in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NOSIP as an antibody target. Whether an autoantibody or antibody against NOSIP could matter depends on whether native NOSIP is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NOSIP is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NOSIP as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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