Seroatlas · Human Serome Atlas

NOL6

Nucleolar protein 6

Also known as: bA311H10.1, FLJ21959, MGC14896, MGC14921, MGC20838, NOL6_HUMAN, Nrap, UTP22

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9H6R4
Gene: NOL6
Ensembl: ENSG00000165271
Chromosome: 9
Canonical length: 1146 aa
Protein class: Predicted intracellular proteins, Predicted membrane proteins
Subcellular location: Nucleoplasm,Nucleoli

OverviewNCBI Gene

The nucleolus is a dense subnuclear membraneless organelle that assembles around clusters of rRNA genes and functions in ribosome biogenesis. This gene encodes a nucleolar RNA-associated protein that is highly conserved between species. RNase treatment of permeabilized cells indicates that the nucleolar localization is RNA dependent. Further studies suggest that the protein is associated with ribosome biogenesis through an interaction with pre-rRNA primary transcripts. Alternative splicing has been observed at this locus and two splice variants encoding distinct isoforms have been identified. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

1146 residues, UniProt reviewed canonical sequence.

>Q9H6R4|NOL6
     1  MGPAPAGEQL RGATGEPEVM EPALEGTGKE GKKASSRKRT LAEPPAKGLL QPVKLSRAEL
    61  YKEPTNEELN RLRETEILFH SSLLRLQVEE LLKEVRLSEK KKDRIDAFLR EVNQRVVRVP
   121  SVPETELTDQ AWLPAGVRVP LHQVPYAVKG CFRFLPPAQV TVVGSYLLGT CIRPDINVDV
   181  ALTMPREILQ DKDGLNQRYF RKRALYLAHL AHHLAQDPLF GSVCFSYTNG CHLKPSLLLR
   241  PRGKDERLVT VRLHPCPPPD FFRPCRLLPT KNNVRSAWYR GQSPAGDGSP EPPTPRYNTW
   301  VLQDTVLESH LQLLSTILSS AQGLKDGVAL LKVWLRQREL DKGQGGFTGF LVSMLVVFLV
   361  STRKIHTTMS GYQVLRSVLQ FLATTDLTVN GISLCLSSDP SLPALADFHQ AFSVVFLDSS
   421  GHLNLCADVT ASTYHQVQHE ARLSMMLLDS RADDGFHLLL MTPKPMIRAF DHVLHLRPLS
   481  RLQAACHRLK LWPELQDNGG DYVSAALGPL TTLLEQGLGA RLNLLAHSRP PVPEWDISQD
   541  PPKHKDSGTL TLGLLLRPEG LTSVLELGPE ADQPEAAKFR QFWGSRSELR RFQDGAIREA
   601  VVWEAASMSQ KRLIPHQVVT HLLALHADIP ETCVHYVGGP LDALIQGLKE TSSTGEEALV
   661  AAVRCYDDLS RLLWGLEGLP LTVSAVQGAH PVLRYTEVFP PTPVRPAFSF YETLRERSSL
   721  LPRLDKPCPA YVEPMTVVCH LEGSGQWPQD AEAVQRVRAA FQLRLAELLT QQHGLQCRAT
   781  ATHTDVLKDG FVFRIRVAYQ REPQILKEVQ SPEGMISLRD TAASLRLERD TRQLPLLTSA
   841  LHGLQQQHPA FSGVARLAKR WVRAQLLGEG FADESLDLVA AALFLHPEPF TPPSSPQVGF
   901  LRFLFLVSTF DWKNNPLFVN LNNELTVEEQ VEIRSGFLAA RAQLPVMVIV TPQDRKNSVW
   961  TQDGPSAQIL QQLVVLAAEA LPMLEKQLMD PRGPGDIRTV FRPPLDIYDV LIRLSPRHIP
  1021  RHRQAVDSPA ASFCRGLLSQ PGPSSLMPVL GYDPPQLYLT QLREAFGDLA LFFYDQHGGE
  1081  VIGVLWKPTS FQPQPFKASS TKGRMVMSRG GELVMVPNVE AILEDFAVLG EGLVQTVEAR
  1141  SERWTV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NOL6 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.26
Highest tissue expression: 29 nTPM

Expression across tissuesHPA

Tissue

skeletal muscle: 29 nTPM
esophagus: 28 nTPM
testis: 27 nTPM
pancreas: 27 nTPM
skin: 26 nTPM
liver: 25 nTPM

Single-cell type

early spermatids: 37 nCPM
late spermatids: 30 nCPM
suprabasal keratinocytes: 24 nCPM
basal keratinocytes: 21 nCPM
esophageal basal cells: 20 nCPM
alveolar cells type 1: 20 nCPM

Immune cell

non-classical monocyte: 6.3 nTPM
intermediate monocyte: 5.9 nTPM
myeloid DC: 5.1 nTPM
MAIT T-cell: 4.1 nTPM
gdT-cell: 3.6 nTPM
memory CD8 T-cell: 3.6 nTPM

Brain region

cerebral cortex: 23 nTPM
hippocampal formation: 20 nTPM
thalamus: 20 nTPM
amygdala: 19 nTPM
white matter: 18 nTPM
basal ganglia: 18 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.18
gnomAD pLI: 1
gnomAD missense Z: 0.86
DepMap mean gene effect: -1.14
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

RNA binding

Cellular components

Protein domainsUniProt · Pfam · InterPro

NOL6/Upt22
Nrap protein domain 1
Nrap protein, domain 2
Nrap protein, domain 3
Nrap protein, domain 4
Nrap protein, domain 5
Nrap protein, domain 6
Nrap protein domain 1
Nrap protein PAP/OAS-like domain
Nrap protein domain 3
Nrap protein nucleotidyltransferase domain 4
Nrap protein PAP/OAS1-like domain 5
Nrap protein domain 6

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NOL6 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

RRP7A

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NOL6 as an antibody target. Whether an autoantibody or antibody against NOL6 could matter depends on whether native NOL6 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NOL6 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NOL6 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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