NMT1

Protein identityUniProt · HPA

UniProt accession

P30419

Gene

NMT1

Ensembl

ENSG00000136448

Chromosome

17

Canonical length

496 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Plasma membrane,Cytosol

OverviewNCBI Gene

Myristate, a rare 14-carbon saturated fatty acid, is cotranslationally attached by an amide linkage to the N-terminal glycine residue of cellular and viral proteins with diverse functions. N-myristoyltransferase (NMT; EC 2.3.1.97) catalyzes the transfer of myristate from CoA to proteins. N-myristoylation appears to be irreversible and is required for full expression of the biologic activities of several N-myristoylated proteins, including the alpha subunit of the signal-transducing guanine nucleotide-binding protein (G protein) GO (GNAO1; MIM 139311) (Duronio et al., 1992 [PubMed 1570339]).[supplied by OMIM, Nov 2008]

Canonical amino-acid sequenceUniProt

496 residues, UniProt reviewed canonical sequence.

>P30419|NMT1
     1  MADESETAVK PPAPPLPQMM EGNGNGHEHC SDCENEEDNS YNRGGLSPAN DTGAKKKKKK
    61  QKKKKEKGSE TDSAQDQPVK MNSLPAERIQ EIQKAIELFS VGQGPAKTME EASKRSYQFW
   121  DTQPVPKLGE VVNTHGPVEP DKDNIRQEPY TLPQGFTWDA LDLGDRGVLK ELYTLLNENY
   181  VEDDDNMFRF DYSPEFLLWA LRPPGWLPQW HCGVRVVSSR KLVGFISAIP ANIHIYDTEK
   241  KMVEINFLCV HKKLRSKRVA PVLIREITRR VHLEGIFQAV YTAGVVLPKP VGTCRYWHRS
   301  LNPRKLIEVK FSHLSRNMTM QRTMKLYRLP ETPKTAGLRP METKDIPVVH QLLTRYLKQF
   361  HLTPVMSQEE VEHWFYPQEN IIDTFVVENA NGEVTDFLSF YTLPSTIMNH PTHKSLKAAY
   421  SFYNVHTQTP LLDLMSDALV LAKMKGFDVF NALDLMENKT FLEKLKFGIG DGNLQYYLYN
   481  WKCPSMGAEK VGLVLQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NMT1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.33

Highest tissue expression

45 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.27

gnomAD pLI

1

gnomAD missense Z

2.48

DepMap mean gene effect

-0.7

DepMap dependency class

common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• in utero embryonic development
• ketone metabolic process
• N-terminal peptidyl-glycine N-myristoylation
• positive regulation of establishment of protein localization to mitochondrion
• protein localization to membrane
• regulation of opsin-mediated signaling pathway

Molecular functions

• glycylpeptide N-tetradecanoyltransferase activity
• peptidyl-lysine N6-myristoyltransferase activity
• myristoyltransferase activity

Cellular components

• cytoplasm
• cytosol
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• Glycylpeptide N-tetradecanoyltransferase
• Acyl-CoA N-acyltransferase
• Glycylpeptide N-tetradecanoyltransferase, N-terminal
• Glycylpeptide N-tetradecanoyltransferase, C-terminal
• Glycylpeptide N-tetradecanoyltransferase, conserved site
• Myristoyl-CoA:protein N-myristoyltransferase, N-terminal domain
• Myristoyl-CoA:protein N-myristoyltransferase, C-terminal domain

KeywordsUniProt

• Acyltransferase
• Cytoplasm
• Membrane
• Phosphoprotein
• Transferase

InteractionsUniProt · HPA

Protein binding partners of NMT1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• ACBD6