Seroatlas · Human Serome Atlas

NME8

Thioredoxin domain-containing protein 3

Also known as: CILD6, DNAI8, NM23-H8, SPTRX2, TXND3_HUMAN, TXNDC3

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8N427
Gene: NME8
Ensembl: ENSG00000086288
Chromosome: 7
Canonical length: 588 aa
Protein class: Disease related genes, Human disease related genes, Predicted intracellular proteins
Subcellular location: Nuclear speckles,Cytosol

OverviewNCBI Gene

This gene encodes a protein with an N-terminal thioredoxin domain and three C-terminal nucleoside diphosphate kinase (NDK) domains, but the NDK domains are thought to be catalytically inactive. The sea urchin ortholog of this gene encodes a component of sperm outer dynein arms, and the protein is implicated in ciliary function. Mutations in this gene are implicated in primary ciliary dyskinesia type 6.[provided by RefSeq, Nov 2009]

Canonical amino-acid sequenceUniProt

588 residues, UniProt reviewed canonical sequence.

>Q8N427|NME8
     1  MASKKREVQL QTVINNQSLW DEMLQNKGLT VIDVYQAWCG PCRAMQPLFR KLKNELNEDE
    61  ILHFAVAEAD NIVTLQPFRD KCEPVFLFSV NGKIIEKIQG ANAPLVNKKV INLIDEERKI
   121  AAGEMARPQY PEIPLVDSDS EVSEESPCES VQELYSIAII KPDAVISKKV LEIKRKITKA
   181  GFIIEAEHKT VLTEEQVVNF YSRIADQCDF EEFVSFMTSG LSYILVVSQG SKHNPPSEET
   241  EPQTDTEPNE RSEDQPEVEA QVTPGMMKNK QDSLQEYLER QHLAQLCDIE EDAANVAKFM
   301  DAFFPDFKKM KSMKLEKTLA LLRPNLFHER KDDVLRIIKD EDFKILEQRQ VVLSEKEAQA
   361  LCKEYENEDY FNKLIENMTS GPSLALVLLR DNGLQYWKQL LGPRTVEEAI EYFPESLCAQ
   421  FAMDSLPVNQ LYGSDSLETA EREIQHFFPL QSTLGLIKPH ATSEQREQIL KIVKEAGFDL
   481  TQVKKMFLTP EQIEKIYPKV TGKDFYKDLL EMLSVGPSMV MILTKWNAVA EWRRLMGPTD
   541  PEEAKLLSPD SIRAQFGISK LKNIVHGASN AYEAKEVVNR LFEDPEEN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NME8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.32
Highest tissue expression: 12 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 12 nTPM
testis: 9.7 nTPM
spleen: 1 nTPM
appendix: 0.6 nTPM
lymph node: 0.5 nTPM
gallbladder: 0.4 nTPM

Single-cell type

late primary spermatocytes: 75 nCPM
early spermatids: 34 nCPM
late spermatids: 28 nCPM
pdcs: 7.1 nCPM
neutrophils: 6.5 nCPM
mast cells: 4.4 nCPM

Immune cell

plasmacytoid DC: 4.7 nTPM
neutrophil: 2.1 nTPM
classical monocyte: 1.1 nTPM
eosinophil: 0.9 nTPM
myeloid DC: 0.9 nTPM
memory CD8 T-cell: 0.7 nTPM

Brain region

medulla oblongata: 2 nTPM
white matter: 1.7 nTPM
pons: 1.6 nTPM
thalamus: 1.5 nTPM
basal ganglia: 1.4 nTPM
spinal cord: 1.2 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about NME8.

Disease | AllUniProt

Conditions NME8 is implicated in, by any mechanism.

Ciliary dyskinesia, primary, 6 (CILD6) MIM:610852

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.34
gnomAD pLI: 0
gnomAD missense Z: -0.38
DepMap mean gene effect: -0.06
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NME8 as an antibody target. Whether an autoantibody or antibody against NME8 could matter depends on whether native NME8 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NME8 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NME8 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Loading the interactive Seroatlas protein explorer...