NKX6-3
Homeobox protein Nkx-6.3
Also known as: FLJ25169, NKX63_HUMAN
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- A6NJ46
- Gene
- NKX6-3
- Ensembl
- ENSG00000165066
- Chromosome
- 8
- Canonical length
- 265 aa
- Protein class
- Predicted intracellular proteins, Transcription factors
OverviewNCBI Gene
The NKX family of homeodomain proteins controls numerous developmental processes. Members of the NKX6 subfamily, including NKX6-3, are involved in development of the central nervous system (CNS), gastrointestinal tract, and pancreas (Alanentalo et al., 2006 [PubMed 16326147]).[supplied by OMIM, Mar 2008]
Canonical amino-acid sequenceUniProt
265 residues, UniProt reviewed canonical sequence.
>A6NJ46|NKX6-3
1 MESNLQGTFL LNNTPLAQFP EMKAPVCQYS VQNSFYKLSP PGLGPQLAAG TPHGITDILS
61 RPVAAPNNSL LSGYPHVAGF GGLSSQGVYY SPQVGNFSKA GNEYPTRTRN CWADTGQDWR
121 GGRQCSNTPD PLSDSIHKKK HTRPTFTGHQ IFALEKTFEQ TKYLAGPERA RLAYSLGMTE
181 SQVKVWFQNR RTKWRKKSAL EPSSSTPRAP GGAGAGAGGD RAPSENEDDE YNKPLDPDSD
241 DEKIRLLLRK HRAAFSVLSL GAHSVLocalizationUniProt · AlphaFold · HPA
Whether an antibody against NKX6-3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.63
- Highest tissue expression
- 27 nTPM
Expression across tissuesHPA
Tissue
- stomach: 27 nTPM
- duodenum: 10 nTPM
- cerebellum: 9.8 nTPM
- parathyroid gland: 1.7 nTPM
- lymph node: 1.4 nTPM
- cerebral cortex: 1 nTPM
Single-cell type
- gastric progenitor cells: 5.1 nCPM
- retinal amacrine cells: 2.8 nCPM
- mucous neck cells: 1.2 nCPM
- foveolar cells: 1.1 nCPM
- pancreatic islet cells: 0.9 nCPM
- brain inhibitory neurons: 0.8 nCPM
Immune cell
- naive B-cell: 0.1 nTPM
- basophil: 0 nTPM
- classical monocyte: 0 nTPM
- eosinophil: 0 nTPM
- gdT-cell: 0 nTPM
- intermediate monocyte: 0 nTPM
Brain region
- cerebellum: 4.6 nTPM
- cerebral cortex: 1.5 nTPM
- midbrain: 1.3 nTPM
- pons: 0.7 nTPM
- white matter: 0.5 nTPM
- medulla oblongata: 0.4 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 1.57
- gnomAD pLI
- 0.02
- gnomAD missense Z
- -0.11
- DepMap mean gene effect
- 0.01
- DepMap dependency class
- selective
OntologyGO
Biological processes
- cell differentiation
- cell fate determination
- glandular epithelial cell differentiation
- negative regulation of stomach neuroendocrine cell differentiation
- negative regulation of transcription by RNA polymerase II
- positive regulation of transcription by RNA polymerase II
- regulation of transcription by RNA polymerase II
- stomach neuroendocrine cell differentiation
Molecular functions
- DNA-binding transcription activator activity, RNA polymerase II-specific
- DNA-binding transcription factor activity, RNA polymerase II-specific
- DNA-binding transcription repressor activity, RNA polymerase II-specific
- RNA polymerase II cis-regulatory region sequence-specific DNA binding
- sequence-specific DNA binding
- sequence-specific double-stranded DNA binding
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads NKX6-3 as an antibody target. Whether an autoantibody or antibody against NKX6-3 could matter depends on whether native NKX6-3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
NKX6-3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label NKX6-3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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