NKX6-1
Homeobox protein Nkx-6.1
Also known as: Nkx6.1, NKX61_HUMAN, NKX6A
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- P78426
- Gene
- NKX6-1
- Ensembl
- ENSG00000163623
- Chromosome
- 4
- Canonical length
- 367 aa
- Protein class
- Predicted intracellular proteins, Transcription factors
- Subcellular location
- Nucleoplasm
OverviewNCBI Gene
In the pancreas, NKX6.1 is required for the development of beta cells and is a potent bifunctional transcription regulator that binds to AT-rich sequences within the promoter region of target genes Iype et al. (2004) [PubMed 15056733].[supplied by OMIM, Mar 2008]
Canonical amino-acid sequenceUniProt
367 residues, UniProt reviewed canonical sequence.
>P78426|NKX6-1
1 MLAVGAMEGT RQSAFLLSSP PLAALHSMAE MKTPLYPAAY PPLPAGPPSS SSSSSSSSSP
61 SPPLGTHNPG GLKPPATGGL SSLGSPPQQL SAATPHGIND ILSRPSMPVA SGAALPSASP
121 SGSSSSSSSS ASASSASAAA AAAAAAAAAA SSPAGLLAGL PRFSSLSPPP PPPGLYFSPS
181 AAAVAAVGRY PKPLAELPGR TPIFWPGVMQ SPPWRDARLA CTPHQGSILL DKDGKRKHTR
241 PTFSGQQIFA LEKTFEQTKY LAGPERARLA YSLGMTESQV KVWFQNRRTK WRKKHAAEMA
301 TAKKKQDSET ERLKGASENE EEDDDYNKPL DPNSDDEKIT QLLKKHKSSS GGGGGLLLHA
361 SEPESSSLocalizationUniProt · AlphaFold · HPA
Whether an antibody against NKX6-1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.65
- Highest tissue expression
- 8.4 nTPM
Expression across tissuesHPA
Tissue
- esophagus: 8.4 nTPM
- pancreas: 6.1 nTPM
- thyroid gland: 3 nTPM
- midbrain: 2.6 nTPM
- testis: 2.1 nTPM
- hypothalamus: 1.3 nTPM
Single-cell type
- late primary spermatocytes: 42 nCPM
- pancreatic islet cells: 41 nCPM
- pancreatic duct cells: 25 nCPM
- ependymal cells: 9.3 nCPM
- fibroblasts: 4 nCPM
- early spermatids: 3 nCPM
Immune cell
- basophil: 0 nTPM
- classical monocyte: 0 nTPM
- eosinophil: 0 nTPM
- gdT-cell: 0 nTPM
- intermediate monocyte: 0 nTPM
- MAIT T-cell: 0 nTPM
Brain region
- midbrain: 31 nTPM
- medulla oblongata: 8.9 nTPM
- spinal cord: 8.2 nTPM
- pons: 8 nTPM
- hypothalamus: 5.7 nTPM
- thalamus: 3 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 1
- gnomAD pLI
- 0.06
- gnomAD missense Z
- 1.08
- DepMap mean gene effect
- 0.14
- DepMap dependency class
- none
OntologyGO
Biological processes
- animal organ morphogenesis
- cell differentiation
- cellular response to cytokine stimulus
- cellular response to peptide hormone stimulus
- glucose mediated signaling pathway
- negative regulation of oligodendrocyte differentiation
- negative regulation of transcription by RNA polymerase II
- oligodendrocyte differentiation
- pancreas development
- pancreatic A cell differentiation
- positive regulation of insulin secretion
- positive regulation of neuron differentiation
- positive regulation of oligodendrocyte differentiation
- positive regulation of transcription by RNA polymerase II
- positive regulation of type B pancreatic cell development
- regulation of axon extension
- regulation of neuron migration
- regulation of transcription by RNA polymerase II
- response to nicotine
- response to xenobiotic stimulus
- smoothened signaling pathway
- spinal cord motor neuron cell fate specification
- transcription by RNA polymerase II
- type B pancreatic cell maturation
- type B pancreatic cell proliferation
Molecular functions
- chromatin binding
- DNA-binding transcription activator activity, RNA polymerase II-specific
- DNA-binding transcription factor activity, RNA polymerase II-specific
- DNA-binding transcription repressor activity, RNA polymerase II-specific
- RNA polymerase II cis-regulatory region sequence-specific DNA binding
- RNA polymerase II-specific DNA-binding transcription factor binding
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads NKX6-1 as an antibody target. Whether an autoantibody or antibody against NKX6-1 could matter depends on whether native NKX6-1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
NKX6-1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label NKX6-1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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