NKX2-8
Homeobox protein Nkx-2.8
Also known as: Nkx2-9, NKX2.8, NKX28_HUMAN, NKX2H
Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene
Protein identityUniProt · HPA
- UniProt accession
- O15522
- Gene
- NKX2-8
- Ensembl
- ENSG00000136327
- Chromosome
- 14
- Canonical length
- 239 aa
- Protein class
- Plasma proteins, Predicted intracellular proteins, Transcription factors
- Subcellular location
- Nucleoplasm,Plasma membrane
OverviewNCBI Gene
The protein encoded by this gene is a homeobox-containing developmental regulator associated with liver development. The encoded protein binds to the alpha-fetoprotein (AFP) gene promoter and increases the expression of AFP. This gene is overexpressed in some lung cancers and is linked to poor patient survival, possibly due to its resistance to cisplatin. This gene is aberrantly methylated in pancreatic cancer, deleted in squamous cell lung carcinomas, and acts as a tumor suppressor in esophageal cancer. Mutations in this gene may also be a cause of neural tube defects. [provided by RefSeq, Dec 2015]
Canonical amino-acid sequenceUniProt
239 residues, UniProt reviewed canonical sequence.
>O15522|NKX2-8
1 MATSGRLSFT VRSLLDLPEQ DAQHLPRREP EPRAPQPDPC AAWLDSERGH YPSSDESSLE
61 TSPPDSSQRP SARPASPGSD AEKRKKRRVL FSKAQTLELE RRFRQQRYLS APEREQLASL
121 LRLTPTQVKI WFQNHRYKLK RARAPGAAES PDLAASAELH AAPGLLRRVV VPVLVRDGQP
181 CGGGGGGEVG TAAAQEKCGA PPAAACPLPG YPAFGPGSAL GLFPAYQHLA SPALVSWNWLocalizationUniProt · AlphaFold · HPA
Whether an antibody against NKX2-8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.
- Antibody reachability
- Intracellular
- Secreted
- No
- Transmembrane segments
- 0
- Mean surface accessibility (rSASA)
- 0.64
- Highest tissue expression
- 5.1 nTPM
Expression across tissuesHPA
Tissue
- spinal cord: 5.1 nTPM
- testis: 5.1 nTPM
- esophagus: 1.6 nTPM
- hypothalamus: 1 nTPM
- midbrain: 0.9 nTPM
- thyroid gland: 0.9 nTPM
Single-cell type
- esophageal apical cells: 21 nCPM
- sertoli cells: 11 nCPM
- respiratory ionocytes: 10 nCPM
- esophageal suprabasal cells: 7.8 nCPM
- esophageal basal cells: 7.3 nCPM
- oocytes: 4.6 nCPM
Immune cell
- basophil: 0 nTPM
- classical monocyte: 0 nTPM
- eosinophil: 0 nTPM
- gdT-cell: 0 nTPM
- intermediate monocyte: 0 nTPM
- MAIT T-cell: 0 nTPM
Brain region
- white matter: 9.2 nTPM
- pons: 5.1 nTPM
- medulla oblongata: 5 nTPM
- hypothalamus: 4.5 nTPM
- spinal cord: 4.2 nTPM
- thalamus: 3.7 nTPM
Genetic constraint and essentialitygnomAD · DepMap
Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.
- gnomAD LOEUF (loss-of-function intolerance)
- 1.52
- gnomAD pLI
- 0
- gnomAD missense Z
- -0.5
- DepMap mean gene effect
- 0.1
- DepMap dependency class
- none
OntologyGO
Biological processes
- axonogenesis
- cell differentiation
- DNA-templated transcription
- epithelial cell proliferation
- liver development
- lung development
- negative regulation of epithelial cell proliferation
- positive regulation of transcription by RNA polymerase II
- regulation of transcription by RNA polymerase II
- transcription by RNA polymerase II
Molecular functions
- DNA-binding transcription activator activity, RNA polymerase II-specific
- DNA-binding transcription factor activity
- DNA-binding transcription factor activity, RNA polymerase II-specific
- double-stranded DNA binding
- RNA polymerase II cis-regulatory region sequence-specific DNA binding
- sequence-specific DNA binding
- sequence-specific double-stranded DNA binding
Cellular components
Protein domainsUniProt · Pfam · InterPro
KeywordsUniProt
Antibody and autoantibody relevanceSeroatlas analysis
Seroatlas reads NKX2-8 as an antibody target. Whether an autoantibody or antibody against NKX2-8 could matter depends on whether native NKX2-8 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.
NKX2-8 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.
Annotation status
The present source text does not explicitly label NKX2-8 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.
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