Seroatlas · Human Serome Atlas

NKX2-4

Homeobox protein Nkx-2.4

Also known as: NKX2.4, NKX24_HUMAN, NKX2D

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9H2Z4
Gene: NKX2-4
Ensembl: ENSG00000125816
Chromosome: 20
Canonical length: 354 aa
Protein class: Predicted intracellular proteins, Transcription factors
Subcellular location: Nuclear bodies

OverviewNCBI Gene

Predicted to enable DNA-binding transcription factor activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Predicted to be involved in cell differentiation and regulation of transcription by RNA polymerase II. Predicted to be located in chromatin. Predicted to be active in nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

354 residues, UniProt reviewed canonical sequence.

>Q9H2Z4|NKX2-4
     1  MSLSPKHTTP FSVSDILSPI EETYKKFSGA MDGAPPGLGA PLGAAAAYRA PPPGPSSQAA
    61  TVAGMQPSHA MAGHNAAAAA AAAAAAAAAA ATYHMPPGVS QFPHGAMGSY CNGGLGNMGE
   121  LPAYTDGMRG GAATGWYGAN PDPRYSSISR FMGPSAGVNV AGMGSLTGIA DAAKSLGPLH
   181  AAAAAAAPRR KRRVLFSQAQ VYELERRFKQ QKYLSAPERE HLASMIHLTP TQVKIWFQNH
   241  RYKMKRQAKD KAAQQLQQEG GLGPPPPPPP SPRRVAVPVL VKDGKPCQNG ASTPTPGQAG
   301  PQPPAPTPAP ELEELSPSPP ALHGPGGGLA ALDAAAGEYS GGVLGANLLY GRTW

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NKX2-4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.68
Highest tissue expression: 1.1 nTPM

Expression across tissuesHPA

Tissue

testis: 1.1 nTPM
hypothalamus: 0.7 nTPM
pituitary gland: 0.2 nTPM
adipose tissue: 0 nTPM
adrenal gland: 0 nTPM
amygdala: 0 nTPM

Single-cell type

early primary spermatocytes: 3.9 nCPM
late spermatids: 3.2 nCPM
early spermatids: 1.6 nCPM
undifferentiated spermatogonia: 1.2 nCPM
differentiating spermatogonia: 0.7 nCPM
late primary spermatocytes: 0.7 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

hypothalamus: 8.8 nTPM
thalamus: 1.1 nTPM
midbrain: 0.6 nTPM
basal ganglia: 0.3 nTPM
medulla oblongata: 0.3 nTPM
pons: 0.3 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.55
gnomAD pLI: 0
gnomAD missense Z: -0.08
DepMap mean gene effect: 0.07
DepMap dependency class: selective

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NKX2-4 as an antibody target. Whether an autoantibody or antibody against NKX2-4 could matter depends on whether native NKX2-4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NKX2-4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NKX2-4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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