NEMF

Protein identityUniProt · HPA

UniProt accession

O60524

Gene

NEMF

Ensembl

ENSG00000165525

Chromosome

14

Canonical length

1076 aa

Protein class

Disease related genes, Human disease related genes, Plasma proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Nuclear bodies,Cytosol

OverviewNCBI Gene

This gene encodes a component of the ribosome quality control complex. The encoded protein facilitates the recognition and ubiquitination of stalled 60S subunits by the ubiquitin ligase listerin. A similar protein in fly functions as a tumor suppressor. [provided by RefSeq, Jul 2016]

Canonical amino-acid sequenceUniProt

1076 residues, UniProt reviewed canonical sequence.

>O60524|NEMF
     1  MKSRFSTIDL RAVLAELNAS LLGMRVNNVY DVDNKTYLIR LQKPDFKATL LLESGIRIHT
    61  TEFEWPKNMM PSSFAMKCRK HLKSRRLVSA KQLGVDRIVD FQFGSDEAAY HLIIELYDRG
   121  NIVLTDYEYV ILNILRFRTD EADDVKFAVR ERYPLDHARA AEPLLTLERL TEIVASAPKG
   181  ELLKRVLNPL LPYGPALIEH CLLENGFSGN VKVDEKLETK DIEKVLVSLQ KAEDYMKTTS
   241  NFSGKGYIIQ KREIKPSLEA DKPVEDILTY EEFHPFLFSQ HSQCPYIEFE SFDKAVDEFY
   301  SKIEGQKIDL KALQQEKQAL KKLDNVRKDH ENRLEALQQA QEIDKLKGEL IEMNLQIVDR
   361  AIQVVRSALA NQIDWTEIGL IVKEAQAQGD PVASAIKELK LQTNHVTMLL RNPYLLSEEE
   421  DDDVDGDVNV EKNETEPPKG KKKKQKNKQL QKPQKNKPLL VDVDLSLSAY ANAKKYYDHK
   481  RYAAKKTQKT VEAAEKAFKS AEKKTKQTLK EVQTVTSIQK ARKVYWFEKF LWFISSENYL
   541  IIGGRDQQQN EIIVKRYLTP GDIYVHADLH GATSCVIKNP TGEPIPPRTL TEAGTMALCY
   601  SAAWDARVIT SAWWVYHHQV SKTAPTGEYL TTGSFMIRGK KNFLPPSYLM MGFSFLFKVD
   661  ESCVWRHQGE RKVRVQDEDM ETLASCTSEL ISEEMEQLDG GDTSSDEDKE EHETPVEVEL
   721  MTQVDQEDIT LQSGRDELNE ELIQEESSED EGEYEEVRKD QDSVGEMKDE GEETLNYPDT
   781  TIDLSHLQPQ RSIQKLASKE ESSNSSDSKS QSRRHLSAKE RREMKKKKLP SDSGDLEALE
   841  GKDKEKESTV HIETHQNTSK NVAAVQPMKR GQKSKMKKMK EKYKDQDEED RELIMKLLGS
   901  AGSNKEEKGK KGKKGKTKDE PVKKQPQKPR GGQRVSDNIK KETPFLEVIT HELQDFAVDD
   961  PHDDKEEQDL DQQGNEENLF DSLTGQPHPE DVLLFAIPIC APYTTMTNYK YKVKLTPGVQ
  1021  KKGKAAKTAL NSFMHSKEAT AREKDLFRSV KDTDLSRNIP GKVKVSAPNL LNVKRK

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NEMF can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.41

Highest tissue expression

40 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about NEMF.

Disease | AllUniProt

Conditions NEMF is implicated in, by any mechanism.

• Intellectual developmental disorder with speech delay and axonal peripheral neuropathy (IDDSAPN) MIM:619099

Disease | GeneticClinVar

29 pathogenic / likely-pathogenic of 233 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

• Intellectual developmental disorder with speech delay and axonal peripheral neuropathy
• See cases
• Inborn genetic diseases
• NEMF-related disorder

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.42

gnomAD pLI

0

gnomAD missense Z

1.19

DepMap mean gene effect

-0.18

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• nuclear export
• protein-containing complex assembly
• rescue of stalled ribosome
• ribosome-associated ubiquitin-dependent protein catabolic process
• CAT tailing

Molecular functions

• alpha-aminoacyl-tRNA binding
• ribosomal large subunit binding
• tRNA binding

Cellular components

• cytosol
• cytosolic ribosome
• nucleus
• RQC complex

Protein domainsUniProt · Pfam · InterPro

• NFACT, RNA-binding domain
• NFACT protein RNA binding domain
• NFACT protein, C-terminal
• Ribosome Quality Control Complex Subunit NEMF
• NFACT N-terminal and middle domains
• NFACT protein C-terminal domain

KeywordsUniProt

• Coiled coil
• Cytoplasm
• Intellectual disability
• Neuropathy
• Nucleus
• Phosphoprotein

InteractionsUniProt · HPA

Protein binding partners of NEMF in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• XPO1
• RPL11