Seroatlas · Human Serome Atlas

NECAB1

N-terminal EF-hand calcium-binding protein 1

Also known as: EFCBP1, NECA1_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q8N987
Gene: NECAB1
Ensembl: ENSG00000123119
Chromosome: 8
Canonical length: 351 aa
Protein class: Predicted intracellular proteins, Transporters
Subcellular location: Nucleoplasm,Primary cilium,Cytosol

OverviewNCBI Gene

Enables identical protein binding activity. Predicted to be involved in regulation of amyloid precursor protein biosynthetic process. Predicted to act upstream of or within blastocyst hatching. Located in cilium; cytosol; and nucleoplasm. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

351 residues, UniProt reviewed canonical sequence.

>Q8N987|NECAB1
     1  MEDSQETSPS SNNSSEELSS ALHLSKGMSI FLDILRRADK NDDGKLSFEE FKAYFADGVL
    61  SGEELHELFH TIDTHNTNNL DTEELCEYFS QHLGEYENVL AALEDLNLSI LKAMGKTKKD
   121  YQEASNLEQF VTRFLLKETL NQLQSLQNSL ECAMETTEEQ TRQERQGPAK PEVLSIQWPG
   181  KRSSRRVQRH NSFSPNSPQF NVSGPGLLEE DNQWMTQINR LQKLIDRLEK KDLKLEPPEE
   241  EIIEGNTKSH IMLVQRQMSV IEEDLEEFQL ALKHYVESAS SQSGCLRISI QKLSNESRYM
   301  IYEFWENSSV WNSHLQTNYS KTFQRSNVDF LETPELTSTM LVPASWWILN N

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NECAB1 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.44
Highest tissue expression: 50 nTPM

Expression across tissuesHPA

Tissue

cerebral cortex: 50 nTPM
spinal cord: 37 nTPM
amygdala: 32 nTPM
hippocampal formation: 30 nTPM
basal ganglia: 27 nTPM
hypothalamus: 17 nTPM

Single-cell type

choroid plexus epithelial cells: 566 nCPM
podocytes: 454 nCPM
oligodendrocytes: 361 nCPM
cardiomyocytes: 354 nCPM
pituicytes/fscs: 268 nCPM
brain inhibitory neurons: 251 nCPM

Immune cell

plasmacytoid DC: 0.2 nTPM
naive CD8 T-cell: 0.1 nTPM
T-reg: 0.1 nTPM
basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM

Brain region

cerebral cortex: 112 nTPM
white matter: 102 nTPM
thalamus: 74 nTPM
basal ganglia: 72 nTPM
choroid plexus: 69 nTPM
hypothalamus: 60 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.58
gnomAD pLI: 0.03
gnomAD missense Z: 1.14
DepMap mean gene effect: 0.17
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NECAB1 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

STX1

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NECAB1 as an antibody target. Whether an autoantibody or antibody against NECAB1 could matter depends on whether native NECAB1 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NECAB1 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NECAB1 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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