Seroatlas · Human Serome Atlas

NDST4

Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 4

Also known as: NDST4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9H3R1
Gene: NDST4
Ensembl: ENSG00000138653
Chromosome: 4
Canonical length: 872 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins

OverviewNCBI Gene

Predicted to enable deacetylase activity and heparan sulfate N-sulfotransferase activity. Predicted to be involved in heparan sulfate proteoglycan biosynthetic process and heparin proteoglycan biosynthetic process. Predicted to be located in Golgi membrane. Predicted to be active in Golgi apparatus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

872 residues, UniProt reviewed canonical sequence.

>Q9H3R1|NDST4
     1  MNLIVKLRRS FRTLIVLLAT FCLVSIVISA YFLYSGYKQE MTLIETTAEA ECTDIKILPY
    61  RSMELKTVKP IDTSKTDPTV LLFVESQYSQ LGQDIIAILE SSRFQYHMVI APGKGDIPPL
   121  TDNGKGKYTL VIYENILKYV SMDSWNRELL EKYCVEYSVS IIGFHKANEN SLPSTQLKGF
   181  PLNLFNNLAL KDCFVNPQSP LLHITKAPKV EKGPLPGEDW TIFQYNHSTY QPVLLTELQT
   241  EKSLSSLSSK TLFATVIQDL GLHDGIQRVL FGNNLNFWLH KLIFIDAISF LSGKRLTLSL
   301  DRYILVDIDD IFVGKEGTRM NVKDVKALLE TQNLLRTQVA NFTFNLGFSG KFYHTGTEEE
   361  DEGDDLLLRS VDEFWWFPHM WSHMQPHLFH NESSLVEQMI LNKEFALEHG IPINMGYAVA
   421  PHHSGVYPVH IQLYAAWKKV WGIQVTSTEE YPHLKPARYR KGFIHNSIMV LPRQTCGLFT
   481  HTIFYKEYPG GPQELDKSIR GGELFLTILL NPISIFMTHL SNYGNDRLGL YTFVNLVNFV
   541  QSWTNLKLQT LPPVQLAHQY FELFPEQKDP LWQNPCDDKR HKDIWSREKT CDHLPKFLVI
   601  GPQKTGTTAL YLFLLMHPSI ISNLPSPKTF EEVQFFNGNN YHKGIDWYMD FFPTPSNTTS
   661  DFLFEKSANY FHSEEAPRRA ASLVPKAKII TILIDPSDRA YSWYQHQRSH EDPAALRFNF
   721  YEVISTGHWA PSDLKTLQRR CLVPGWYAVH IERWLTYFAT SQLLIIDGQQ LRSDPATVMD
   781  EVQKFLGVTP RYNYSEALTF DPQKGFWCQL LEGGKTKCLG KSKGRKYPPM DPESRTFLSN
   841  YYRDHNVELS KLLHRLGQPL PSWLRQELQK VR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NDST4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 1
Mean surface accessibility (rSASA): 0.25
Highest tissue expression: 0.8 nTPM

Expression across tissuesHPA

Tissue

cerebral cortex: 0.8 nTPM
ovary: 0.8 nTPM
spleen: 0.7 nTPM
endometrium: 0.4 nTPM
smooth muscle: 0.4 nTPM
cervix: 0.3 nTPM

Single-cell type

thymic myoid cells: 114 nCPM
thyrotrophs: 70 nCPM
brain inhibitory neurons: 55 nCPM
other brain neurons: 54 nCPM
lactotrophs: 31 nCPM
brain excitatory neurons: 29 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

pons: 20 nTPM
medulla oblongata: 9.7 nTPM
midbrain: 8.1 nTPM
hypothalamus: 5.5 nTPM
thalamus: 3.4 nTPM
basal ganglia: 2.9 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.64
gnomAD pLI: 0
gnomAD missense Z: 1.09
DepMap mean gene effect: 0.06
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NDST4 as an antibody target. Whether an autoantibody or antibody against NDST4 could matter depends on whether native NDST4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NDST4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NDST4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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