Seroatlas · Human Serome Atlas

NDST3

Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 3

Also known as: HSST3, NDST3_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
O95803
Gene
NDST3
Ensembl
ENSG00000164100
Chromosome
4
Canonical length
873 aa
Protein class
Enzymes, Metabolic proteins, Predicted intracellular proteins

OverviewNCBI Gene

This gene encodes a member of the heparan sulfate/heparin GlcNAc N-deacetylase/ N-sulfotransferase family. The encoded enzyme is a type II transmembrane protein that resides in the Golgi apparatus. This monomeric bifunctional enzyme catalyzes the N-deacetylation and N-sulfation of N-acetylglucosamine residues in heparan sulfate and heparin, which are the initial chemical modifications required for the biosynthesis of the functional oligosaccharide sequences that define the specific ligand binding activities of heparan sulfate and heparin. [provided by RefSeq, Nov 2008]

Canonical amino-acid sequenceUniProt

873 residues, UniProt reviewed canonical sequence.

>O95803|NDST3
     1  MSFIMKLHRH FQRTVILLAT FCMVSIIISA YYLYSGYKQE NELSETASEV DCGDLQHLPY
    61  QLMEVKAMKL FDASRTDPTV LVFVESQYSS LGQDIIMILE SSRFQYHIEI APGKGDLPVL
   121  IDKMKGKYIL IIYENILKYI NMDSWNRSLL DKYCVEYGVG VIGFHKTSEK SVQSFQLKGF
   181  PFSIYGNLAV KDCCINPHSP LIRVTKSSKL EKGSLPGTDW TVFQINHSAY QPVIFAKVKT
   241  PENLSPSISK GAFYATIIHD LGLHDGIQRV LFGNNLNFWL HKLIFIDAIS FLSGKRLTLS
   301  LDRYILVDID DIFVGKEGTR MNTNDVKALL DTQNLLRAQI TNFTFNLGFS GKFYHTGTEE
   361  EDEGDDCLLG SVDEFWWFPH MWSHMQPHLF HNESSLVEQM ILNKKFALEH GIPTDMGYAV
   421  APHHSGVYPV HVQLYEAWKK VWNIKITSTE EYPHLKPARY RRGFIHKNIM VLPRQTCGLF
   481  THTIFYKEYP GGPKELDKSI QGGELFFTVV LNPISIFMTH LSNYGNDRLG LYTFVNLANF
   541  VKSWTNLRLQ TLPPVQLAHK YFELFPDQKD PLWQNPCDDK RHRDIWSKEK TCDRLPKFLV
   601  IGPQKTGTTA LYLFLVMHPS ILSNSPSPKT FEEVQFFNRN NYHRGIDWYM DFFPVPSNVT
   661  TDFLFEKSAN YFHSEEAPKR AASLVPKAKI ITILIDPSDR AYSWYQHQRS HEDPAALKFS
   721  FYEVISAGPR APSELRALQK RCLVPGWYAS HIERWLVYFP PFQLLIIDGQ QLRTDPATVM
   781  DEVQKFLGVL PHYNYSEALT FDSHKGFWCQ LLEEGKTKCL GKSKGRKYPP MDSDSRTFLS
   841  SYYRDHNVEL SKLLHKLGQP LPSWLRQELQ KVR

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NDST3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
1
Mean surface accessibility (rSASA)
0.25
Highest tissue expression
5.1 nTPM

Expression across tissuesHPA

Tissue

  • cerebellum: 5.1 nTPM
  • basal ganglia: 3.8 nTPM
  • cerebral cortex: 3 nTPM
  • thymus: 2.7 nTPM
  • retina: 2.5 nTPM
  • spleen: 1.9 nTPM

Single-cell type

  • kupffer cells: 519 nCPM
  • brain inhibitory neurons: 339 nCPM
  • brain excitatory neurons: 311 nCPM
  • retinal bipolar cells: 254 nCPM
  • thymocytes: 134 nCPM
  • lactotrophs: 121 nCPM

Immune cell

  • basophil: 0.1 nTPM
  • classical monocyte: 0 nTPM
  • eosinophil: 0 nTPM
  • gdT-cell: 0 nTPM
  • intermediate monocyte: 0 nTPM
  • MAIT T-cell: 0 nTPM

Brain region

  • basal ganglia: 18 nTPM
  • cerebellum: 15 nTPM
  • cerebral cortex: 14 nTPM
  • hippocampal formation: 9.6 nTPM
  • hypothalamus: 9.1 nTPM
  • amygdala: 8.4 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.59
gnomAD pLI
0
gnomAD missense Z
2.59
DepMap mean gene effect
0.13
DepMap dependency class
none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NDST3 as an antibody target. Whether an autoantibody or antibody against NDST3 could matter depends on whether native NDST3 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NDST3 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NDST3 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/NDST3. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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