Seroatlas · Human Serome Atlas

NCKAP5L

Nck-associated protein 5-like

Also known as: KIAA1602, NCK5L_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q9HCH0
Gene: NCKAP5L
Ensembl: ENSG00000167566
Chromosome: 12
Canonical length: 1334 aa
Protein class: Predicted intracellular proteins
Quaternary structure: Homodimer

OverviewNCBI Gene

Involved in microtubule bundle formation and microtubule depolymerization. Located in centrosome and microtubule plus-end. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

1334 residues, UniProt reviewed canonical sequence.

>Q9HCH0|NCKAP5L
     1  MSEAMDQPAG GPGNPRPGEG DDGSMEPGTC QELLHRLREL EAENSALAQA NENQRETYER
    61  CLDEVANHVV QALLNQKDLR EECIKLKKRV FDLERQNQML SALFQQKLQL TTGSLPQIPL
   121  TPLQPPSEPP ASPSLSSTEG PAAPLPLGHC AGQREVCWEQ QLRPGGPGPP AAPPPALDAL
   181  SPFLRKKAQI LEVLRALEET DPLLLCSPAT PWRPPGQGPG SPEPINGELC GPPQPEPSPW
   241  APCLLLGPGN LGGLLHWERL LGGLGGEEDT GRPWGPSRGP PQAQGTSSGP NCAPGSSSSS
   301  SSDEAGDPNE APSPDTLLGA LARRQLNLGQ LLEDTESYLQ AFLAGAAGPL NGDHPGPGQS
   361  SSPDQAPPQL SKSKGLPKSA WGGGTPEAHR PGFGATSEGQ GPLPFLSMFM GAGDAPLGSR
   421  PGHPHSSSQV KSKLQIGPPS PGEAQGPLLP SPARGLKFLK LPPTSEKSPS PGGPQLSPQL
   481  PRNSRIPCRN SGSDGSPSPL LARRGLGGGE LSPEGAQGLP TSPSPCYTTP DSTQLRPPQS
   541  ALSTTLSPGP VVSPCYENIL DLSRSTFRGP SPEPPPSPLQ VPTYPQLTLE VPQAPEVLRS
   601  PGVPPSPCLP ESYPYGSPQE KSLDKAGSES PHPGRRTPGN SSKKPSQGSG RRPGDPGSTP
   661  LRDRLAALGK LKTGPEGALG SEKNGVPARP GTEKTRGPGK SGESAGDMVP SIHRPLEQLE
   721  AKGGIRGAVA LGTNSLKQQE PGLMGDPGAR VYSSHSMGAR VDLEPVSPRS CLTKVELAKS
   781  RLAGALCPQV PRTPAKVPTS APSLGKPNKS PHSSPTKLPS KSPTKVVPRP GAPLVTKESP
   841  KPDKGKGPPW ADCGSTTAQS TPLVPGPTDP SQGPEGLAPH SAIEEKVMKG IEENVLRLQG
   901  QERAPGAEVK HRNTSSIASW FGLKKSKLPA LNRRTEATKN KEGAGGGSPL RREVKMEARK
   961  LEAESLNISK LMAKAEDLRR ALEEEKAYLS SRARPRPGGP APGPNTGLGQ VQGQLAGMYQ
  1021  GADTFMQQLL NRVDGKELPS KSWREPKPEY GDFQPVSSDP KSPWPACGPR NGLVGPLQGC
  1081  GKPPGKPSSE PGRREEMPSE DSLAEPVPTS HFTACGSLTR TLDSGIGTFP PPDHGSSGTP
  1141  SKNLPKTKPP RLDPPPGVPP ARPPPLTKVP RRAHTLEREV PGIEELLVSG RHPSMPAFPA
  1201  LLPAAPGHRG HETCPDDPCE DPGPTPPVQL AKNWTFPNTR AAGSSSDPLM CPPRQLEGLP
  1261  RTPMALPVDR KRSQEPSRPS PTPQGPPFGG SRTPSTSDMA EEGRVASGGP PGLETSESLS
  1321  DSLYDSLSSC GSQG

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NCKAP5L can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.69
Highest tissue expression: 27 nTPM

Expression across tissuesHPA

Tissue

adrenal gland: 27 nTPM
heart muscle: 21 nTPM
lung: 16 nTPM
testis: 16 nTPM
cerebellum: 15 nTPM
prostate: 14 nTPM

Single-cell type

peritubular myoid cells: 126 nCPM
leydig cells: 94 nCPM
cone photoreceptor cells: 77 nCPM
epididymal basal cells: 75 nCPM
granulosa cells: 70 nCPM
adipocytes: 67 nCPM

Immune cell

classical monocyte: 0.6 nTPM
myeloid DC: 0.5 nTPM
plasmacytoid DC: 0.5 nTPM
intermediate monocyte: 0.4 nTPM
non-classical monocyte: 0.4 nTPM
total PBMC: 0.3 nTPM

Brain region

white matter: 33 nTPM
medulla oblongata: 30 nTPM
cerebral cortex: 30 nTPM
thalamus: 28 nTPM
basal ganglia: 26 nTPM
pons: 26 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.37
gnomAD pLI: 0.63
gnomAD missense Z: 0.74
DepMap mean gene effect: -0.17
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of NCKAP5L in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads NCKAP5L as an antibody target. Whether an autoantibody or antibody against NCKAP5L could matter depends on whether native NCKAP5L is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

NCKAP5L is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label NCKAP5L as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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