NAMPT

Protein identityUniProt · HPA

UniProt accession

P43490

Gene

NAMPT

Ensembl

ENSG00000105835

Chromosome

7

Canonical length

491 aa

Protein class

Cancer-related genes, Enzymes, Metabolic proteins, Plasma proteins, Predicted intracellular proteins, Predicted secreted proteins

Subcellular location

Nuclear speckles,Cell Junctions

Secretome location

Secreted to blood

Quaternary structure

Homodimer

OverviewNCBI Gene

This gene encodes a protein that catalyzes the condensation of nicotinamide with 5-phosphoribosyl-1-pyrophosphate to yield nicotinamide mononucleotide, one step in the biosynthesis of nicotinamide adenine dinucleotide. The protein belongs to the nicotinic acid phosphoribosyltransferase (NAPRTase) family and is thought to be involved in many important biological processes, including metabolism, stress response and aging. This gene has a pseudogene on chromosome 10. [provided by RefSeq, Feb 2011]

Canonical amino-acid sequenceUniProt

491 residues, UniProt reviewed canonical sequence.

>P43490|NAMPT
     1  MNPAAEAEFN ILLATDSYKV THYKQYPPNT SKVYSYFECR EKKTENSKLR KVKYEETVFY
    61  GLQYILNKYL KGKVVTKEKI QEAKDVYKEH FQDDVFNEKG WNYILEKYDG HLPIEIKAVP
   121  EGFVIPRGNV LFTVENTDPE CYWLTNWIET ILVQSWYPIT VATNSREQKK ILAKYLLETS
   181  GNLDGLEYKL HDFGYRGVSS QETAGIGASA HLVNFKGTDT VAGLALIKKY YGTKDPVPGY
   241  SVPAAEHSTI TAWGKDHEKD AFEHIVTQFS SVPVSVVSDS YDIYNACEKI WGEDLRHLIV
   301  SRSTQAPLII RPDSGNPLDT VLKVLEILGK KFPVTENSKG YKLLPPYLRV IQGDGVDINT
   361  LQEIVEGMKQ KMWSIENIAF GSGGGLLQKL TRDLLNCSFK CSYVVTNGLG INVFKDPVAD
   421  PNKRSKKGRL SLHRTPAGNF VTLEEGKGDL EEYGQDLLHT VFKNGKVTKS YSFDEIRKNA
   481  QLNIELEAAH H

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against NAMPT can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Secreted

Secreted

Yes

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.24

Highest tissue expression

339 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.31

gnomAD pLI

0.98

gnomAD missense Z

2.97

DepMap mean gene effect

-0.44

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 8% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• cell-cell signaling
• circadian regulation of gene expression
• inflammatory response
• NAD+ biosynthetic process
• NAD+ biosynthetic process via the salvage pathway
• NADP+ biosynthetic process
• nicotinamide metabolic process
• nicotinate metabolic process
• positive regulation of canonical NF-kappaB signal transduction
• positive regulation of cell population proliferation
• positive regulation of ERK1 and ERK2 cascade
• positive regulation of gene expression
• positive regulation of transcription by RNA polymerase II
• signal transduction

Molecular functions

• cytokine activity
• identical protein binding
• nicotinamide phosphoribosyltransferase activity

Cellular components

• cell junction
• cytosol
• extracellular exosome
• mitochondrial matrix
• nuclear speck

Protein domainsUniProt · Pfam · InterPro

• Aldolase-type TIM barrel
• Nicotinate phosphoribosyltransferase-like, C-terminal
• Nicotinamide phosphoribosyl transferase
• Nicotinate/nicotinamide phosphoribosyltransferase
• Nicotinamide phosphoribosyltransferase, N-terminal domain
• Nicotinate phosphoribosyltransferase (NAPRTase) family
• Nicotinamide phosphoribosyltransferase, N-terminal domain

KeywordsUniProt

• Acetylation
• Biological rhythms
• Cytokine
• Cytoplasm
• Glycosyltransferase
• Nucleus
• Phosphoprotein
• Pyridine nucleotide biosynthesis
• Secreted
• Transferase

InteractionsUniProt · HPA

Protein binding partners of NAMPT in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• FTL
• IFITM3
• MT-ND1