MRPL33

Protein identityUniProt · HPA

UniProt accession

O75394

Gene

MRPL33

Ensembl

ENSG00000243147

Chromosome

2

Canonical length

65 aa

Protein class

Predicted intracellular proteins, Ribosomal proteins

OverviewNCBI Gene

Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

65 residues, UniProt reviewed canonical sequence.

>O75394|MRPL33
     1  MFLSAVFFAK SKSKNILVRM VSEAGTGFCF NTKRNRLREK LTLLHYDPVV KQRVLFVEKK
    61  KIRSL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MRPL33 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.44

Highest tissue expression

672 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

1.01

gnomAD pLI

0.37

gnomAD missense Z

0.37

DepMap mean gene effect

-0.38

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• mitochondrial translation

Molecular functions

• structural constituent of ribosome

Cellular components

• mitochondrial inner membrane
• mitochondrial large ribosomal subunit
• mitochondrion

Protein domainsUniProt · Pfam · InterPro

• Zinc-binding ribosomal protein
• Large ribosomal subunit protein bL33
• Large ribosomal subunit protein bL33 superfamily
• Mitochondrial ribosomal protein bL33
• Ribosomal protein L33

KeywordsUniProt

• Mitochondrion
• Ribonucleoprotein
• Ribosomal protein
• Transit peptide

InteractionsUniProt · HPA

Protein binding partners of MRPL33 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• GADD45GIP1
• MRPL50