Seroatlas · Human Serome Atlas

MISFA

Mitochondrial sheath formation-associated protein

Also known as: Kastor, MISFA_HUMAN, Polluks, SPTY2D1-AS1, SPTY2D1OS

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
A0A0U1RRN3
Gene
MISFA
Ensembl
ENSG00000247595
Chromosome
11
Canonical length
59 aa
Protein class
Predicted intracellular proteins, Predicted membrane proteins
Secretome location
Intracellular and membrane

OverviewNCBI Gene

Predicted to be involved in sperm mitochondrial sheath assembly. Predicted to be located in mitochondrial outer membrane. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

59 residues, UniProt reviewed canonical sequence.

>A0A0U1RRN3|MISFA
     1  MIVLGWMFFV GLVCYMGTFP ELMPPTLKWQ ERWPVQESKT QLRRRALGED LLQNHVEGI

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MISFA can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
1
Mean surface accessibility (rSASA)
0.56
Highest tissue expression
476 nTPM

Expression across tissuesHPA

Tissue

  • testis: 476 nTPM
  • skin: 13 nTPM
  • epididymis: 3.5 nTPM
  • retina: 3.2 nTPM
  • breast: 2.8 nTPM
  • rectum: 2.2 nTPM

Single-cell type

  • late spermatids: 24,677 nCPM
  • early spermatids: 4,241 nCPM
  • late primary spermatocytes: 877 nCPM
  • sertoli cells: 56 nCPM
  • parietal cells: 51 nCPM
  • epicardial cells: 48 nCPM

Immune cell

  • eosinophil: 0.6 nTPM
  • MAIT T-cell: 0.5 nTPM
  • neutrophil: 0.5 nTPM
  • classical monocyte: 0.3 nTPM
  • T-reg: 0.3 nTPM
  • basophil: 0.2 nTPM

Brain region

  • choroid plexus: 14 nTPM
  • cerebellum: 13 nTPM
  • white matter: 12 nTPM
  • medulla oblongata: 11 nTPM
  • cerebral cortex: 11 nTPM
  • hypothalamus: 11 nTPM

OntologyGO

Cellular components

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MISFA in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MISFA as an antibody target. Whether an autoantibody or antibody against MISFA could matter depends on whether native MISFA is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MISFA is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MISFA as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/MISFA. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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