MGAT4B

Protein identityUniProt · HPA

UniProt accession

Q9UQ53

Gene

MGAT4B

Ensembl

ENSG00000161013

Chromosome

5

Canonical length

548 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Golgi apparatus,Vesicles,Cytosol

OverviewNCBI Gene

This gene encodes a key glycosyltransferase that regulates the formation of tri- and multiantennary branching structures in the Golgi apparatus. The encoded protein, in addition to the related isoenzyme A, catalyzes the transfer of N-acetylglucosamine (GlcNAc) from UDP-GlcNAc in a beta-1,4 linkage to the Man-alpha-1,3-Man-beta-1,4-GlcNAc arm of R-Man-alpha-1,6(GlcNAc-beta-1,2-Man-alpha-1,3)Man-beta-1,4-GlcNAc-beta-1,4-GlcNAc-beta-1-Asn. The encoded protein may play a role in regulating the availability of serum glycoproteins, oncogenesis, and differentiation. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

548 residues, UniProt reviewed canonical sequence.

>Q9UQ53|MGAT4B
     1  MRLRNGTFLT LLLFCLCAFL SLSWYAALSG QKGDVVDVYQ REFLALRDRL HAAEQESLKR
    61  SKELNLVLDE IKRAVSERQA LRDGDGNRTW GRLTEDPRLK PWNGSHRHVL HLPTVFHHLP
   121  HLLAKESSLQ PAVRVGQGRT GVSVVMGIPS VRREVHSYLT DTLHSLISEL SPQEKEDSVI
   181  VVLIAETDSQ YTSAVTENIK ALFPTEIHSG LLEVISPSPH FYPDFSRLRE SFGDPKERVR
   241  WRTKQNLDYC FLMMYAQSKG IYYVQLEDDI VAKPNYLSTM KNFALQQPSE DWMILEFSQL
   301  GFIGKMFKSL DLSLIVEFIL MFYRDKPIDW LLDHILWVKV CNPEKDAKHC DRQKANLRIR
   361  FKPSLFQHVG THSSLAGKIQ KLKDKDFGKQ ALRKEHVNPP AEVSTSLKTY QHFTLEKAYL
   421  REDFFWAFTP AAGDFIRFRF FQPLRLERFF FRSGNIEHPE DKLFNTSVEV LPFDNPQSDK
   481  EALQEGRTAT LRYPRSPDGY LQIGSFYKGV AEGEVDPAFG PLEALRLSIQ TDSPVWVILS
   541  EIFLKKAD

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MGAT4B can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.33

Highest tissue expression

281 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.39

gnomAD pLI

0.69

gnomAD missense Z

1.31

DepMap mean gene effect

-0.06

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• glycoprotein biosynthetic process
• N-glycan processing
• protein N-linked glycosylation
• viral protein processing

Molecular functions

• acetylglucosaminyltransferase activity
• alpha-1,3-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity
• metal ion binding

Cellular components

• endoplasmic reticulum
• endoplasmic reticulum-Golgi intermediate compartment
• Golgi membrane
• Golgi stack

Protein domainsUniProt · Pfam · InterPro

• Glycosyl transferase family 54
• MGAT4 A/B/C, C-terminal domain
• MGAT4, conserved region
• MGAT4 conserved region
• MGAT4 C-terminal domain

KeywordsUniProt

• Coiled coil
• Glycoprotein
• Glycosyltransferase
• Golgi apparatus
• Membrane
• Metal-binding
• Signal-anchor
• Transferase
• Transmembrane
• Transmembrane helix

InteractionsUniProt · HPA

Protein binding partners of MGAT4B in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• SLC35A3