MGAT3

Protein identityUniProt · HPA

UniProt accession

Q09327

Gene

MGAT3

Ensembl

ENSG00000128268

Chromosome

22

Canonical length

533 aa

Protein class

Enzymes, Metabolic proteins, Predicted intracellular proteins

OverviewNCBI Gene

There are believed to be over 100 different glycosyltransferases involved in the synthesis of protein-bound and lipid-bound oligosaccharides. The enzyme encoded by this gene transfers a GlcNAc residue to the beta-linked mannose of the trimannosyl core of N-linked oligosaccharides and produces a bisecting GlcNAc. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

533 residues, UniProt reviewed canonical sequence.

>Q09327|MGAT3
     1  MKMRRYKLFL MFCMAGLCLI SFLHFFKTLS YVTFPRELAS LSPNLVSSFF WNNAPVTPQA
    61  SPEPGGPDLL RTPLYSHSPL LQPLPPSKAA EELHRVDLVL PEDTTEYFVR TKAGGVCFKP
   121  GTKMLERPPP GRPEEKPEGA NGSSARRPPR YLLSARERTG GRGARRKWVE CVCLPGWHGP
   181  SCGVPTVVQY SNLPTKERLV PREVPRRVIN AINVNHEFDL LDVRFHELGD VVDAFVVCES
   241  NFTAYGEPRP LKFREMLTNG TFEYIRHKVL YVFLDHFPPG GRQDGWIADD YLRTFLTQDG
   301  VSRLRNLRPD DVFIIDDADE IPARDGVLFL KLYDGWTEPF AFHMRKSLYG FFWKQPGTLE
   361  VVSGCTVDML QAVYGLDGIR LRRRQYYTMP NFRQYENRTG HILVQWSLGS PLHFAGWHCS
   421  WCFTPEGIYF KLVSAQNGDF PRWGDYEDKR DLNYIRGLIR TGGWFDGTQQ EYPPADPSEH
   481  MYAPKYLLKN YDRFHYLLDN PYQEPRSTAA GGWRHRGPEG RPPARGKLDE AEV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MGAT3 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Other membrane

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.36

Highest tissue expression

41 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.63

gnomAD pLI

0.15

gnomAD missense Z

3.13

DepMap mean gene effect

-0.03

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• amyloid-beta metabolic process
• cellular response to oxidative stress
• cognition
• intracellular protein localization
• N-acetylglucosamine metabolic process
• negative regulation of lysosomal protein catabolic process
• positive regulation of protein localization to early endosome
• protein N-linked glycosylation
• regulation of cell migration

Molecular functions

• glycosyltransferase activity
• beta-1,4-mannosylglycoprotein 4-beta-N-acetylglucosaminyltransferase activity

Cellular components

• Golgi membrane
• membrane

Protein domainsUniProt · Pfam · InterPro

• Glycosyl transferase, family 17
• Glycosyltransferase family 17

KeywordsUniProt

• Glycoprotein
• Glycosyltransferase
• Golgi apparatus
• Membrane
• Signal-anchor
• Transferase
• Transmembrane
• Transmembrane helix

InteractionsUniProt · HPA

Protein binding partners of MGAT3 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• MGAT4D