Seroatlas · Human Serome Atlas

METTL16

RNA N(6)-adenosine-methyltransferase METTL16

Also known as: MET16_HUMAN, METT10D, MGC3329

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q86W50
Gene: METTL16
Ensembl: ENSG00000127804
Chromosome: 17
Canonical length: 562 aa
Protein class: Enzymes, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Cytosol

OverviewNCBI Gene

Enables RNA binding activity and RNA methyltransferase activity. Involved in RNA processing and regulation of mRNA metabolic process. Located in cytoplasm and nucleus. [provided by Alliance of Genome Resources, Jul 2025]

Canonical amino-acid sequenceUniProt

562 residues, UniProt reviewed canonical sequence.

>Q86W50|METTL16
     1  MALSKSMHAR NRYKDKPPDF AYLASKYPDF KQHVQINLNG RVSLNFKDPE AVRALTCTLL
    61  REDFGLSIDI PLERLIPTVP LRLNYIHWVE DLIGHQDSDK STLRRGIDIG TGASCIYPLL
   121  GATLNGWYFL ATEVDDMCFN YAKKNVEQNN LSDLIKVVKV PQKTLLMDAL KEESEIIYDF
   181  CMCNPPFFAN QLEAKGVNSR NPRRPPPSSV NTGGITEIMA EGGELEFVKR IIHDSLQLKK
   241  RLRWYSCMLG KKCSLAPLKE ELRIQGVPKV TYTEFCQGRT MRWALAWSFY DDVTVPSPPS
   301  KRRKLEKPRK PITFVVLASV MKELSLKASP LRSETAEGIV VVTTWIEKIL TDLKVQHKRV
   361  PCGKEEVSLF LTAIENSWIH LRRKKRERVR QLREVPRAPE DVIQALEEKK PTPKESGNSQ
   421  ELARGPQERT PCGPALREGE AAAVEGPCPS QESLSQEENP EPTEDERSEE KGGVEVLESC
   481  QGSSNGAQDQ EASEQFGSPV AERGKRLPGV AGQYLFKCLI NVKKEVDDAL VEMHWVEGQN
   541  RDLMNQLCTY IRNQIFRLVA VN

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against METTL16 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.37
Highest tissue expression: 9.7 nTPM

Expression across tissuesHPA

Tissue

ovary: 9.7 nTPM
thymus: 9.7 nTPM
parathyroid gland: 9.5 nTPM
endometrium: 8.9 nTPM
thyroid gland: 8.7 nTPM
fallopian tube: 8.5 nTPM

Single-cell type

myonuclei: 132 nCPM
cone photoreceptor cells: 126 nCPM
rod photoreceptor cells: 113 nCPM
medullary thymic epithelial cells: 110 nCPM
b-cells: 106 nCPM
somatotrophs: 103 nCPM

Immune cell

naive CD4 T-cell: 4.9 nTPM
MAIT T-cell: 4.4 nTPM
naive CD8 T-cell: 3.7 nTPM
gdT-cell: 3.5 nTPM
memory B-cell: 3.5 nTPM
memory CD8 T-cell: 3.4 nTPM

Brain region

white matter: 19 nTPM
basal ganglia: 16 nTPM
midbrain: 14 nTPM
spinal cord: 14 nTPM
cerebellum: 13 nTPM
medulla oblongata: 13 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.41
gnomAD pLI: 0.61
gnomAD missense Z: 2.35
DepMap mean gene effect: -1.06
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

mRNA m(6)A methyltransferase activity
RNA binding
RNA stem-loop binding
U6 snRNA 3'-end binding
23S rRNA (adenine(1618)-N(6))-methyltransferase activity
U6 snRNA (adenine-(43)-N(6))-methyltransferase activity

Cellular components

Protein domainsUniProt · Pfam · InterPro

S-adenosyl-L-methionine-dependent methyltransferase superfamily
METTL16/RlmF family
Methyltransferase METTL16/PsiM
RNA methyltransferase

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of METTL16 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads METTL16 as an antibody target. Whether an autoantibody or antibody against METTL16 could matter depends on whether native METTL16 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

METTL16 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label METTL16 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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