Seroatlas · Human Serome Atlas

MASTL

Serine/threonine-protein kinase greatwall

Also known as: FLJ14813, Gwl, GWL_HUMAN, THC2

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q96GX5
Gene: MASTL
Ensembl: ENSG00000120539
Chromosome: 10
Canonical length: 879 aa
Protein class: Disease related genes, Enzymes, Potential drug targets, Predicted intracellular proteins
Subcellular location: Nucleoplasm

OverviewNCBI Gene

This gene encodes a microtubule-associated serine/threonine kinase. Mutations at this locus have been associated with autosomal dominant thrombocytopenia, also known as thrombocytopenia-2. Alternatively spliced transcript variants have been described for this locus. [provided by RefSeq, Feb 2010]

Canonical amino-acid sequenceUniProt

879 residues, UniProt reviewed canonical sequence.

>Q96GX5|MASTL
     1  MDPTAGSKKE PGGGAATEEG VNRIAVPKPP SIEEFSIVKP ISRGAFGKVY LGQKGGKLYA
    61  VKVVKKADMI NKNMTHQVQA ERDALALSKS PFIVHLYYSL QSANNVYLVM EYLIGGDVKS
   121  LLHIYGYFDE EMAVKYISEV ALALDYLHRH GIIHRDLKPD NMLISNEGHI KLTDFGLSKV
   181  TLNRDINMMD ILTTPSMAKP RQDYSRTPGQ VLSLISSLGF NTPIAEKNQD PANILSACLS
   241  ETSQLSQGLV CPMSVDQKDT TPYSSKLLKS CLETVASNPG MPVKCLTSNL LQSRKRLATS
   301  SASSQSHTFI SSVESECHSS PKWEKDCQES DEALGPTMMS WNAVEKLCAK SANAIETKGF
   361  NKKDLELALS PIHNSSALPT TGRSCVNLAK KCFSGEVSWE AVELDVNNIN MDTDTSQLGF
   421  HQSNQWAVDS GGISEEHLGK RSLKRNFELV DSSPCKKIIQ NKKTCVEYKH NEMTNCYTNQ
   481  NTGLTVEVQD LKLSVHKSQQ NDCANKENIV NSFTDKQQTP EKLPIPMIAK NLMCELDEDC
   541  EKNSKRDYLS SSFLCSDDDR ASKNISMNSD SSFPGISIME SPLESQPLDS DRSIKESSFE
   601  ESNIEDPLIV TPDCQEKTSP KGVENPAVQE SNQKMLGPPL EVLKTLASKR NAVAFRSFNS
   661  HINASNNSEP SRMNMTSLDA MDISCAYSGS YPMAITPTQK RRSCMPHQQT PNQIKSGTPY
   721  RTPKSVRRGV APVDDGRILG TPDYLAPELL LGRAHGPAVD WWALGVCLFE FLTGIPPFND
   781  ETPQQVFQNI LKRDIPWPEG EEKLSDNAQS AVEILLTIDD TKRAGMKELK RHPLFSDVDW
   841  ENLQHQTMPF IPQPDDETDT SYFEARNTAQ HLTVSGFSL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MASTL can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.53
Highest tissue expression: 18 nTPM

Expression across tissuesHPA

Tissue

bone marrow: 18 nTPM
thymus: 9.7 nTPM
lymph node: 8.3 nTPM
tonsil: 6.7 nTPM
placenta: 6.1 nTPM
testis: 6 nTPM

Single-cell type

epicardial cells: 405 nCPM
cardiomyocytes: 86 nCPM
monocyte progenitors: 82 nCPM
extravillous trophoblasts: 61 nCPM
innate lymphoid cells: 54 nCPM
neutrophil progenitors: 50 nCPM

Immune cell

neutrophil: 2.3 nTPM
classical monocyte: 1.8 nTPM
non-classical monocyte: 1.8 nTPM
eosinophil: 1.7 nTPM
intermediate monocyte: 1.7 nTPM
naive B-cell: 1.6 nTPM

Brain region

white matter: 5.9 nTPM
cerebellum: 5.8 nTPM
choroid plexus: 5.7 nTPM
pons: 5.7 nTPM
thalamus: 5.7 nTPM
cerebral cortex: 5.5 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.95
gnomAD pLI: 0
gnomAD missense Z: 0.5
DepMap mean gene effect: -1.23
DepMap dependency class: common

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 6% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

Protein kinase domain
AGC-kinase, C-terminal
Serine/threonine-protein kinase, active site
Protein kinase-like domain superfamily
Serine/threonine-protein kinases, AGC
Protein kinase domain
Serine/threonine-protein kinase greatwall, catalytic domain

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MASTL as an antibody target. Whether an autoantibody or antibody against MASTL could matter depends on whether native MASTL is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MASTL is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MASTL as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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