Seroatlas · Human Serome Atlas

MAST4

Microtubule-associated serine/threonine-protein kinase 4

Also known as: KIAA0303, MAST4_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: O15021
Gene: MAST4
Ensembl: ENSG00000069020
Chromosome: 5
Canonical length: 2623 aa
Protein class: Enzymes, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Cytosol

OverviewNCBI Gene

This gene encodes a member of the microtubule-associated serine/threonine protein kinases. The proteins in this family contain a domain that gives the kinase the ability to determine its own scaffold to control the effects of their kinase activities. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2014]

Canonical amino-acid sequenceUniProt

2623 residues, UniProt reviewed canonical sequence.

>O15021|MAST4
     1  MGEKVSEAPE PVPRGCSGHG SRTPASALVA ASSPGASSAE SSSGSETLSE EGEPGGFSRE
    61  HQPPPPPPLG GTLGARAPAA WAPASVLLER GVLALPPPLP GGAVPPAPRG SSASQEEQDE
   121  ELDHILSPPP MPFRKCSNPD VASGPGKSLK YKRQLSEDGR QLRRGSLGGA LTGRYLLPNP
   181  VAGQAWPASA ETSNLVRMRS QALGQSAPSL TASLKELSLP RRGSFCRTSN RKSLIGNGQS
   241  PALPRPHSPL SAHAGNSPQD SPRNFSPSAS AHFSFARRTD GRRWSLASLP SSGYGTNTPS
   301  STVSSSCSSQ EKLHQLPYQP TPDELHFLSK HFCTTESIAT ENRCRNTPMR PRSRSLSPGR
   361  SPACCDHEII MMNHVYKERF PKATAQMEER LKEIITSYSP DNVLPLADGV LSFTHHQIIE
   421  LARDCLDKSH QGLITSRYFL ELQHKLDKLL QEAHDRSESG ELAFIKQLVR KILIVIARPA
   481  RLLECLEFDP EEFYYLLEAA EGHAKEGQGI KTDIPRYIIS QLGLNKDPLE EMAHLGNYDS
   541  GTAETPETDE SVSSSNASLK LRRKPRESDF ETIKLISNGA YGAVYFVRHK ESRQRFAMKK
   601  INKQNLILRN QIQQAFVERD ILTFAENPFV VSMYCSFETR RHLCMVMEYV EGGDCATLMK
   661  NMGPLPVDMA RMYFAETVLA LEYLHNYGIV HRDLKPDNLL VTSMGHIKLT DFGLSKVGLM
   721  SMTTNLYEGH IEKDAREFLD KQVCGTPEYI APEVILRQGY GKPVDWWAMG IILYEFLVGC
   781  VPFFGDTPEE LFGQVISDEI NWPEKDEAPP PDAQDLITLL LRQNPLERLG TGGAYEVKQH
   841  RFFRSLDWNS LLRQKAEFIP QLESEDDTSY FDTRSEKYHH METEEEDDTN DEDFNVEIRQ
   901  FSSCSHRFSK VFSSIDRITQ NSAEEKEDSV DKTKSTTLPS TETLSWSSEY SEMQQLSTSN
   961  SSDTESNRHK LSSGLLPKLA ISTEGEQDEA ASCPGDPHEE PGKPALPPEE CAQEEPEVTT
  1021  PASTISSSTL SVGSFSEHLD QINGRSECVD STDNSSKPSS EPASHMARQR LESTEKKKIS
  1081  GKVTKSLSAS ALSLMIPGDM FAVSPLGSPM SPHSLSSDPS SSRDSSPSRD SSAASASPHQ
  1141  PIVIHSSGKN YGFTIRAIRV YVGDSDIYTV HHIVWNVEEG SPACQAGLKA GDLITHINGE
  1201  PVHGLVHTEV IELLLKSGNK VSITTTPFEN TSIKTGPARR NSYKSRMVRR SKKSKKKESL
  1261  ERRRSLFKKL AKQPSPLLHT SRSFSCLNRS LSSGESLPGS PTHSLSPRSP TPSYRSTPDF
  1321  PSGTNSSQSS SPSSSAPNSP AGSGHIRPST LHGLAPKLGG QRYRSGRRKS AGNIPLSPLA
  1381  RTPSPTPQPT SPQRSPSPLL GHSLGNSKIA QAFPSKMHSP PTIVRHIVRP KSAEPPRSPL
  1441  LKRVQSEEKL SPSYGSDKKH LCSRKHSLEV TQEEVQREQS QREAPLQSLD ENVCDVPPLS
  1501  RARPVEQGCL KRPVSRKVGR QESVDDLDRD KLKAKVVVKK ADGFPEKQES HQKSHGPGSD
  1561  LENFALFKLE EREKKVYPKA VERSSTFENK ASMQEAPPLG SLLKDALHKQ ASVRASEGAM
  1621  SDGRVPAEHR QGGGDFRRAP APGTLQDGLC HSLDRGISGK GEGTEKSSQA KELLRCEKLD
  1681  SKLANIDYLR KKMSLEDKED NLCPVLKPKM TAGSHECLPG NPVRPTGGQQ EPPPASESRA
  1741  FVSSTHAAQM SAVSFVPLKA LTGRVDSGTE KPGLVAPESP VRKSPSEYKL EGRSVSCLKP
  1801  IEGTLDIALL SGPQASKTEL PSPESAQSPS PSGDVRASVP PVLPSSSGKK NDTTSARELS
  1861  PSSLKMNKSY LLEPWFLPPS RGLQNSPAVS LPDPEFKRDR KGPHPTARSP GTVMESNPQQ
  1921  REGSSPKHQD HTTDPKLLTC LGQNLHSPDL ARPRCPLPPE ASPSREKPGL RESSERGPPT
  1981  ARSERSAARA DTCREPSMEL CFPETAKTSD NSKNLLSVGR THPDFYTQTQ AMEKAWAPGG
  2041  KTNHKDGPGE ARPPPRDNSS LHSAGIPCEK ELGKVRRGVE PKPEALLARR SLQPPGIESE
  2101  KSEKLSSFPS LQKDGAKEPE RKEQPLQRHP SSIPPPPLTA KDLSSPAARQ HCSSPSHASG
  2161  REPGAKPSTA EPSSSPQDPP KPVAAHSESS SHKPRPGPDP GPPKTKHPDR SLSSQKPSVG
  2221  ATKGKEPATQ SLGGSSREGK GHSKSGPDVF PATPGSQNKA SDGIGQGEGG PSVPLHTDRA
  2281  PLDAKPQPTS GGRPLEVLEK PVHLPRPGHP GPSEPADQKL SAVGEKQTLS PKHPKPSTVK
  2341  DCPTLCKQTD NRQTDKSPSQ PAANTDRRAE GKKCTEALYA PAEGDKLEAG LSFVHSENRL
  2401  KGAERPAAGV GKGFPEARGK GPGPQKPPTE ADKPNGMKRS PSATGQSSFR STALPEKSLS
  2461  CSSSFPETRA GVREASAASS DTSSAKAAGG MLELPAPSNR DHRKAQPAGE GRTHMTKSDS
  2521  LPSFRVSTLP LESHHPDPNT MGGASHRDRA LSVTATVGET KGKDPAPAQP PPARKQNVGR
  2581  DVTKPSPAPN TDRPISLSNE KDFVVRQRRG KESLRSSPHK KAL

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MAST4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.57
Highest tissue expression: 28 nTPM

Expression across tissuesHPA

Tissue

heart muscle: 28 nTPM
esophagus: 26 nTPM
vagina: 20 nTPM
cervix: 16 nTPM
thymus: 16 nTPM
urinary bladder: 15 nTPM

Single-cell type

urothelial cells: 2,795 nCPM
cardiomyocytes: 1,833 nCPM
proximal tubule cells: 1,393 nCPM
suprabasal keratinocytes: 1,245 nCPM
epididymal basal cells: 1,183 nCPM
epicardial cells: 1,146 nCPM

Immune cell

basophil: 15 nTPM
T-reg: 6.9 nTPM
naive CD4 T-cell: 4.2 nTPM
memory CD4 T-cell: 4 nTPM
neutrophil: 3.4 nTPM
memory B-cell: 2.9 nTPM

Brain region

cerebral cortex: 119 nTPM
white matter: 84 nTPM
midbrain: 74 nTPM
medulla oblongata: 72 nTPM
amygdala: 71 nTPM
hypothalamus: 70 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about MAST4.

Disease | GeneticClinVar

3 pathogenic / likely-pathogenic of 407 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

Infantile spasms
Moyamoya angiopathy

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.38
gnomAD pLI: 0
gnomAD missense Z: 1.93
DepMap mean gene effect: -0.08
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MAST4 as an antibody target. Whether an autoantibody or antibody against MAST4 could matter depends on whether native MAST4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MAST4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MAST4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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