Seroatlas · Human Serome Atlas

MARCHF8

E3 ubiquitin-protein ligase MARCHF8

Also known as: c-MIR, CMIR, MARCH-VIII, MARCH8, MARH8_HUMAN, MIR, RNF178

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: Q5T0T0
Gene: MARCHF8
Ensembl: ENSG00000165406
Chromosome: 10
Canonical length: 291 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins, Predicted membrane proteins

OverviewNCBI Gene

MARCH8 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). MARCH enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their vesicular transport between membrane compartments. MARCH8 induces the internalization of several membrane glycoproteins (Goto et al., 2003 [PubMed 12582153]; Bartee et al., 2004 [PubMed 14722266]).[supplied by OMIM, Apr 2010]

Canonical amino-acid sequenceUniProt

291 residues, UniProt reviewed canonical sequence.

>Q5T0T0|MARCHF8
     1  MSMPLHQISA IPSQDAISAR VYRSKTKEKE REEQNEKTLG HFMSHSSNIS KAGSPPSASA
    61  PAPVSSFSRT SITPSSQDIC RICHCEGDDE SPLITPCHCT GSLHFVHQAC LQQWIKSSDT
   121  RCCELCKYEF IMETKLKPLR KWEKLQMTSS ERRKIMCSVT FHVIAITCVV WSLYVLIDRT
   181  AEEIKQGQAT GILEWPFWTK LVVVAIGFTG GLLFMYVQCK VYVQLWKRLK AYNRVIYVQN
   241  CPETSKKNIF EKSPLTEPNF ENKHGYGICH SDTNSSCCTE PEDTGAEIIH V

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MARCHF8 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Other membrane
Secreted: No
Transmembrane segments: 2
Mean surface accessibility (rSASA): 0.51
Highest tissue expression: 53 nTPM

Expression across tissuesHPA

Tissue

testis: 53 nTPM
bone marrow: 46 nTPM
pancreas: 25 nTPM
cerebellum: 21 nTPM
small intestine: 21 nTPM
colon: 20 nTPM

Single-cell type

early spermatids: 393 nCPM
cone photoreceptor cells: 292 nCPM
late spermatids: 165 nCPM
b-cells: 123 nCPM
choroid plexus epithelial cells: 112 nCPM
oligodendrocytes: 108 nCPM

Immune cell

neutrophil: 14 nTPM
basophil: 14 nTPM
eosinophil: 11 nTPM
NK-cell: 8.5 nTPM
naive B-cell: 5.4 nTPM
MAIT T-cell: 4.5 nTPM

Brain region

white matter: 72 nTPM
medulla oblongata: 59 nTPM
cerebellum: 58 nTPM
basal ganglia: 56 nTPM
pons: 53 nTPM
thalamus: 52 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 1.31
gnomAD pLI: 0
DepMap mean gene effect: -0.08
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MARCHF8 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

CD86

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MARCHF8 as an antibody target. Whether an autoantibody or antibody against MARCHF8 could matter depends on whether native MARCHF8 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MARCHF8 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MARCHF8 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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