Seroatlas · Human Serome Atlas

MARCHF11

E3 ubiquitin-protein ligase MARCHF11

Also known as: MARCH-XI, MARCH11, MARHB_HUMAN, RNF226

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: A6NNE9
Gene: MARCHF11
Ensembl: ENSG00000183654
Chromosome: 5
Canonical length: 402 aa
Protein class: Enzymes, Metabolic proteins, Predicted intracellular proteins, Predicted membrane proteins

OverviewNCBI Gene

MARCH11 is a member of the MARCH family of membrane-bound E3 ubiquitin ligases (EC 6.3.2.19). These enzymes add ubiquitin (see MIM 191339) to target lysines in substrate proteins, thereby signaling their intracellular transport. March11 appears to have a role in ubiquitin-mediated protein sorting in the trans-Golgi network (TGN)-multivesicular body (MVB) transport pathway (Morokuma et al., 2007 [PubMed 17604280]).[supplied by OMIM, Apr 2010]

Canonical amino-acid sequenceUniProt

402 residues, UniProt reviewed canonical sequence.

>A6NNE9|MARCHF11
     1  MSFEGGHGGS RCRGAESGDA EPPPQPPPPP PPTPPPGEPA PVPAAPRYLP PLPASPETPE
    61  RAAGPSEPLG EVAPRCRGAD ELPPPPLPLQ PAGQEVAAAG DSGEGPRRLP EAAAAKGGPG
   121  ESEAGAGGER ERRGAGDQPE TRSVCSSRSS SSGGGDQRAG HQHQHHQPIC KICFQGAEQG
   181  ELLNPCRCDG SVRYTHQLCL LKWISERGSW TCELCCYRYH VIAIKMKQPC QWQSISITLV
   241  EKVQMIAVIL GSLFLIASVT WLLWSAFSPY AVWQRKDILF QICYGMYGFM DLVCIGLIVH
   301  EGAAVYRVFK RWRAVNLHWD VLNYDKATDI EESSRGESST SRTLWLPLTA LRNRNLVHPT
   361  QLTSPRFQCG YVLLHLFNRM RPHEDLSEDN SSGEVVMRVT SV

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MARCHF11 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 2
Mean surface accessibility (rSASA): 0.57
Highest tissue expression: 79 nTPM

Expression across tissuesHPA

Tissue

testis: 79 nTPM
pituitary gland: 9.5 nTPM
hypothalamus: 6 nTPM
cerebral cortex: 5.9 nTPM
cerebellum: 5.7 nTPM
heart muscle: 4.6 nTPM

Single-cell type

cardiomyocytes: 612 nCPM
thyrotrophs: 503 nCPM
somatotrophs: 355 nCPM
retinal ganglion cells: 305 nCPM
late primary spermatocytes: 164 nCPM
early spermatids: 163 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

hypothalamus: 8.7 nTPM
cerebral cortex: 8.1 nTPM
medulla oblongata: 6.5 nTPM
pons: 5 nTPM
cerebellum: 4.9 nTPM
basal ganglia: 4.4 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.93
gnomAD pLI: 0
DepMap mean gene effect: -0.01
DepMap dependency class: none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

protein ubiquitination

Molecular functions

Cellular components

cytoplasmic vesicle membrane

Protein domainsUniProt · Pfam · InterPro

Zinc finger, RING-CH-type
Zinc finger, RING/FYVE/PHD-type
E3 ubiquitin-protein ligase MARCHF4/9/11
RING-variant domain
MARCHF11, RING-CH finger C4HC3-type

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MARCHF11 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

AP1M1

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MARCHF11 as an antibody target. Whether an autoantibody or antibody against MARCHF11 could matter depends on whether native MARCHF11 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MARCHF11 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MARCHF11 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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