Seroatlas · Human Serome Atlas

MAPKAPK5

MAP kinase-activated protein kinase 5

Also known as: MAPK5_HUMAN, MK5, PRAK

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q8IW41
Gene
MAPKAPK5
Ensembl
ENSG00000089022
Chromosome
12
Canonical length
473 aa
Protein class
Disease related genes, Enzymes, Potential drug targets, Predicted intracellular proteins, Predicted membrane proteins
Subcellular location
Nucleoplasm,Cytosol

OverviewNCBI Gene

The protein encoded by this gene is a tumor suppressor and member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. The encoded protein is found in the nucleus but translocates to the cytoplasm upon phosphorylation and activation. This kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites. Two alternately spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Nov 2012]

Canonical amino-acid sequenceUniProt

473 residues, UniProt reviewed canonical sequence.

>Q8IW41|MAPKAPK5
     1  MSEESDMDKA IKETSILEEY SINWTQKLGA GISGPVRVCV KKSTQERFAL KILLDRPKAR
    61  NEVRLHMMCA THPNIVQIIE VFANSVQFPH ESSPRARLLI VMEMMEGGEL FHRISQHRHF
   121  TEKQASQVTK QIALALRHCH LLNIAHRDLK PENLLFKDNS LDAPVKLCDF GFAKIDQGDL
   181  MTPQFTPYYV APQVLEAQRR HQKEKSGIIP TSPTPYTYNK SCDLWSLGVI IYVMLCGYPP
   241  FYSKHHSRTI PKDMRRKIMT GSFEFPEEEW SQISEMAKDV VRKLLKVKPE ERLTIEGVLD
   301  HPWLNSTEAL DNVLPSAQLM MDKAVVAGIQ QAHAEQLANM RIQDLKVSLK PLHSVNNPIL
   361  RKRKLLGTKP KDSVYIHDHE NGAEDSNVAL EKLRDVIAQC ILPQAGKGEN EDEKLNEVMQ
   421  EAWKYNRECK LLRDTLQSFS WNGRGFTDKV DRLKLAEIVK QVIEEQTTSH ESQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MAPKAPK5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Intracellular
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.32
Highest tissue expression
30 nTPM

Expression across tissuesHPA

Tissue

  • skeletal muscle: 30 nTPM
  • rectum: 29 nTPM
  • choroid plexus: 28 nTPM
  • small intestine: 27 nTPM
  • colon: 26 nTPM
  • bone marrow: 26 nTPM

Single-cell type

  • respiratory ionocytes: 74 nCPM
  • cardiomyocytes: 70 nCPM
  • myonuclei: 65 nCPM
  • enterocytes: 63 nCPM
  • goblet cells: 61 nCPM
  • erythrocyte progenitors: 57 nCPM

Immune cell

  • non-classical monocyte: 8.8 nTPM
  • T-reg: 4.8 nTPM
  • gdT-cell: 4.2 nTPM
  • naive CD4 T-cell: 4.2 nTPM
  • memory B-cell: 3.6 nTPM
  • classical monocyte: 3.3 nTPM

Brain region

  • choroid plexus: 31 nTPM
  • hippocampal formation: 29 nTPM
  • cerebral cortex: 29 nTPM
  • cerebellum: 29 nTPM
  • basal ganglia: 27 nTPM
  • hypothalamus: 27 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about MAPKAPK5.

Disease | AllUniProt

Conditions MAPKAPK5 is implicated in, by any mechanism.

  • Neurocardiofaciodigital syndrome (NCFD) MIM:619869

Disease | GeneticClinVar

6 pathogenic / likely-pathogenic of 87 ClinVar records.

Conditions with pathogenic or likely-pathogenic variants.

  • Neurocardiofaciodigital syndrome

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.45
gnomAD pLI
0.35
gnomAD missense Z
2.42
DepMap mean gene effect
-0.15
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 7% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MAPKAPK5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MAPKAPK5 as an antibody target. Whether an autoantibody or antibody against MAPKAPK5 could matter depends on whether native MAPKAPK5 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MAPKAPK5 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MAPKAPK5 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/MAPKAPK5. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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