Seroatlas · Human Serome Atlas

MAPK4

Mitogen-activated protein kinase 4

Also known as: Erk3-related, Erk4, MK04_HUMAN, PRKM4

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession: P31152
Gene: MAPK4
Ensembl: ENSG00000141639
Chromosome: 18
Canonical length: 587 aa
Protein class: Enzymes, Plasma proteins, Predicted intracellular proteins
Subcellular location: Nucleoplasm,Golgi apparatus,Midbody,Cytosol
Quaternary structure: Homodimer

OverviewNCBI Gene

Mitogen-activated protein kinase 4 is a member of the mitogen-activated protein kinase family. Tyrosine kinase growth factor receptors activate mitogen-activated protein kinases which then translocate into the nucleus and phosphorylate nuclear targets. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Canonical amino-acid sequenceUniProt

587 residues, UniProt reviewed canonical sequence.

>P31152|MAPK4
     1  MAEKGDCIAS VYGYDLGGRF VDFQPLGFGV NGLVLSAVDS RACRKVAVKK IALSDARSMK
    61  HALREIKIIR RLDHDNIVKV YEVLGPKGTD LQGELFKFSV AYIVQEYMET DLARLLEQGT
   121  LAEEHAKLFM YQLLRGLKYI HSANVLHRDL KPANIFISTE DLVLKIGDFG LARIVDQHYS
   181  HKGYLSEGLV TKWYRSPRLL LSPNNYTKAI DMWAAGCILA EMLTGRMLFA GAHELEQMQL
   241  ILETIPVIRE EDKDELLRVM PSFVSSTWEV KRPLRKLLPE VNSEAIDFLE KILTFNPMDR
   301  LTAEMGLQHP YMSPYSCPED EPTSQHPFRI EDEIDDIVLM AANQSQLSNW DTCSSRYPVS
   361  LSSDLEWRPD RCQDASEVQR DPRAGSAPLA EDVQVDPRKD SHSSSERFLE QSHSSMERAF
   421  EADYGRSCDY KVGSPSYLDK LLWRDNKPHH YSEPKLILDL SHWKQAAGAP PTATGLADTG
   481  AREDEPASLF LEIAQWVKST QGGPEHASPP ADDPERRLSA SPPGRPAPVD GGASPQFDLD
   541  VFISRALKLC TKPEDLPDNK LGDLNGACIP EHPGDLVQTE AFSKERW

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MAPK4 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability: Intracellular
Secreted: No
Transmembrane segments: 0
Mean surface accessibility (rSASA): 0.42
Highest tissue expression: 72 nTPM

Expression across tissuesHPA

Tissue

basal ganglia: 72 nTPM
parathyroid gland: 48 nTPM
midbrain: 42 nTPM
amygdala: 39 nTPM
cerebral cortex: 37 nTPM
hypothalamus: 32 nTPM

Single-cell type

pituicytes/fscs: 1,914 nCPM
choroid plexus epithelial cells: 533 nCPM
bergmann glia: 489 nCPM
astrocytes: 388 nCPM
müller glia: 345 nCPM
ependymal cells: 229 nCPM

Immune cell

basophil: 0 nTPM
classical monocyte: 0 nTPM
eosinophil: 0 nTPM
gdT-cell: 0 nTPM
intermediate monocyte: 0 nTPM
MAIT T-cell: 0 nTPM

Brain region

thalamus: 157 nTPM
basal ganglia: 148 nTPM
midbrain: 128 nTPM
medulla oblongata: 121 nTPM
pons: 102 nTPM
hypothalamus: 101 nTPM

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about MAPK4.

Disease | ImmuneIEDB

Conditions an epitope on MAPK4 was assayed in.

melanoma T cell

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance): 0.52
gnomAD pLI: 0.22
gnomAD missense Z: 1.68
DepMap mean gene effect: 0.1
DepMap dependency class: selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MAPK4 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

FASTKD5

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MAPK4 as an antibody target. Whether an autoantibody or antibody against MAPK4 could matter depends on whether native MAPK4 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MAPK4 is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MAPK4 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

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