MAP4K5

Protein identityUniProt · HPA

UniProt accession

Q9Y4K4

Gene

MAP4K5

Ensembl

ENSG00000012983

Chromosome

14

Canonical length

846 aa

Protein class

Enzymes, Predicted intracellular proteins

Subcellular location

Plasma membrane,Primary cilium,Cytosol

OverviewNCBI Gene

This gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

846 residues, UniProt reviewed canonical sequence.

>Q9Y4K4|MAP4K5
     1  MEAPLRPAAD ILRRNPQQDY ELVQRVGSGT YGDVYKARNV HTGELAAVKI IKLEPGDDFS
    61  LIQQEIFMVK ECKHCNIVAY FGSYLSREKL WICMEYCGGG SLQDIYHVTG PLSELQIAYV
   121  CRETLQGLAY LHTKGKMHRD IKGANILLTD HGDVKLADFG VAAKITATIA KRKSFIGTPY
   181  WMAPEVAAVE KNGGYNQLCD IWAVGITAIE LGELQPPMFD LHPMRALFLM SKSNFQPPKL
   241  KDKTKWSSTF HNFVKIALTK NPKKRPTAER LLTHTFVAQP GLSRALAVEL LDKVNNPDNH
   301  AHYTEADDDD FEPHAIIRHT IRSTNRNARA ERTASEINFD KLQFEPPLRK ETEARDEMGL
   361  SSDPNFMLQW NPFVDGANTG KSTSKRAIPP PLPPKPRISS YPEDNFPDEE KASTIKHCPD
   421  SESRAPQILR RQSSPSCGPV AETSSIGNGD GISKLMSENT EGSAQAPQLP RKKDKRDFPK
   481  PAINGLPPTP KVLMGACFSK VFDGCPLKIN CATSWIHPDT KDQYIIFGTE DGIYTLNLNE
   541  LHEATMEQLF PRKCTWLYVI NNTLMSLSEG KTFQLYSHNL IALFEHAKKP GLAAHIQTHR
   601  FPDRILPRKF ALTTKIPDTK GCHKCCIVRN PYTGHKYLCG ALQSGIVLLQ WYEPMQKFML
   661  IKHFDFPLPS PLNVFEMLVI PEQEYPMVCV AISKGTESNQ VVQFETINLN SASSWFTEIG
   721  AGSQQLDSIH VTQLERDTVL VCLDKFVKIV NLQGKLKSSK KLASELSFDF RIESVVCLQD
   781  SVLAFWKHGM QGKSFKSDEV TQEISDETRV FRLLGSDRVV VLESRPTENP TAHSNLYILA
   841  GHENSY

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MAP4K5 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.34

Highest tissue expression

31 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.28

gnomAD pLI

0.99

gnomAD missense Z

2.06

DepMap mean gene effect

0.03

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• intracellular signal transduction

Molecular functions

• ATP binding
• MAP kinase kinase kinase kinase activity
• protein kinase activity
• protein serine kinase activity
• protein serine/threonine kinase activity

Cellular components

• cilium
• cytoplasm
• cytosol
• plasma membrane

Protein domainsUniProt · Pfam · InterPro

• Protein kinase domain
• Citron homology (CNH) domain
• Protein kinase-like domain superfamily
• Protein kinase, ATP binding site
• Mitogen-activated protein (MAP) kinase kinase kinase kinase
• STE20/SPS1-related Proline-Alanine-rich Kinase
• Protein kinase domain
• CNH domain

KeywordsUniProt

• ATP-binding
• Cytoplasm
• Kinase
• Nucleotide-binding
• Phosphoprotein
• Serine/threonine-protein kinase
• Transferase

InteractionsUniProt · HPA

Protein binding partners of MAP4K5 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• CRKL
• CRK
• GRB2
• MAP4K3