Seroatlas · Human Serome Atlas

MAP3K12

Mitogen-activated protein kinase kinase kinase 12

Also known as: DLK, M3K12_HUMAN, MEKK12, MUK, ZPK, ZPKP1

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q12852
Gene
MAP3K12
Ensembl
ENSG00000139625
Chromosome
12
Canonical length
859 aa
Protein class
Enzymes, Predicted intracellular proteins, Transporters
Subcellular location
Nucleoplasm,Plasma membrane
Quaternary structure
Homodimer

OverviewNCBI Gene

This gene encodes a member of the serine/threonine protein kinase family. This kinase contains a leucine-zipper domain and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation. Alternatively spliced transcript variants encoding different proteins have been described.[provided by RefSeq, Jul 2010]

Canonical amino-acid sequenceUniProt

859 residues, UniProt reviewed canonical sequence.

>Q12852|MAP3K12
     1  MACLHETRTP SPSFGGFVST LSEASMRKLD PDTSDCTPEK DLTPTHVLQL HEQDAGGPGG
    61  AAGSPESRAS RVRADEVRLQ CQSGSGFLEG LFGCLRPVWT MIGKAYSTEH KQQQEDLWEV
   121  PFEEILDLQW VGSGAQGAVF LGRFHGEEVA VKKVRDLKET DIKHLRKLKH PNIITFKGVC
   181  TQAPCYCILM EFCAQGQLYE VLRAGRPVTP SLLVDWSMGI AGGMNYLHLH KIIHRDLKSP
   241  NMLITYDDVV KISDFGTSKE LSDKSTKMSF AGTVAWMAPE VIRNEPVSEK VDIWSFGVVL
   301  WELLTGEIPY KDVDSSAIIW GVGSNSLHLP VPSSCPDGFK ILLRQCWNSK PRNRPSFRQI
   361  LLHLDIASAD VLSTPQETYF KSQAEWREEV KLHFEKIKSE GTCLHRLEEE LVMRRREELR
   421  HALDIREHYE RKLERANNLY MELNALMLQL ELKERELLRR EQALERRCPG LLKPHPSRGL
   481  LHGNTMEKLI KKRNVPQKLS PHSKRPDILK TESLLPKLDA ALSGVGLPGC PKGPPSPGRS
   541  RRGKTRHRKA SAKGSCGDLP GLRTAVPPHE PGGPGSPGGL GGGPSAWEAC PPALRGLHHD
   601  LLLRKMSSSS PDLLSAALGS RGRGATGGAG DPGSPPPARG DTPPSEGSAP GSTSPDSPGG
   661  AKGEPPPPVG PGEGVGLLGT GREGTSGRGG SRAGSQHLTP AALLYRAAVT RSQKRGISSE
   721  EEEGEVDSEV ELTSSQRWPQ SLNMRQSLST FSSENPSDGE EGTASEPSPS GTPEVGSTNT
   781  DERPDERSDD MCSQGSEIPL DPPPSEVIPG PEPSSLPIPH QELLRERGPP NSEDSDCDST
   841  ELDNSNSVDA LRPPASLPP

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MAP3K12 can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Cell surface
Secreted
No
Transmembrane segments
0
Mean surface accessibility (rSASA)
0.54
Highest tissue expression
65 nTPM

Expression across tissuesHPA

Tissue

  • cerebellum: 65 nTPM
  • cerebral cortex: 24 nTPM
  • cervix: 20 nTPM
  • pituitary gland: 20 nTPM
  • endometrium: 19 nTPM
  • ovary: 19 nTPM

Single-cell type

  • epicardial cells: 116 nCPM
  • rod photoreceptor cells: 45 nCPM
  • cardiomyocytes: 38 nCPM
  • thymocytes: 34 nCPM
  • retinal amacrine cells: 34 nCPM
  • fibro-adipogenic progenitors: 32 nCPM

Immune cell

  • neutrophil: 1 nTPM
  • MAIT T-cell: 0.8 nTPM
  • memory CD8 T-cell: 0.6 nTPM
  • classical monocyte: 0.5 nTPM
  • memory CD4 T-cell: 0.5 nTPM
  • gdT-cell: 0.4 nTPM

Brain region

  • cerebellum: 63 nTPM
  • cerebral cortex: 45 nTPM
  • white matter: 35 nTPM
  • pons: 35 nTPM
  • thalamus: 35 nTPM
  • hippocampal formation: 34 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.07
gnomAD pLI
1
gnomAD missense Z
2.99
DepMap mean gene effect
-0.09
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 5% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

InteractionsUniProt · HPA

Protein binding partners of MAP3K12 in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MAP3K12 as an antibody target. Whether an autoantibody or antibody against MAP3K12 could matter depends on whether native MAP3K12 is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MAP3K12 is annotated at the cell surface, where native MAP3K12 is exposed to circulating antibodies and is a prime autoantibody target that could block, deplete, or overstimulate it.

Annotation status

The present source text does not explicitly label MAP3K12 as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/MAP3K12. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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