Seroatlas · Human Serome Atlas

MANEA

Glycoprotein endo-alpha-1,2-mannosidase

Also known as: FLJ12838, mandaselin, MANEA_HUMAN

Cross-references: UniProt · Ensembl · Human Protein Atlas · GeneCards · NCBI Gene

Protein identityUniProt · HPA

UniProt accession
Q5SRI9
Gene
MANEA
Ensembl
ENSG00000172469
Chromosome
6
Canonical length
462 aa
Protein class
Enzymes, Predicted intracellular proteins, Predicted membrane proteins
Subcellular location
Golgi apparatus

OverviewNCBI Gene

N-glycosylation of proteins is initiated in the endoplasmic reticulum (ER) by the transfer of the preassembled oligosaccharide glucose-3-mannose-9-N-acetylglucosamine-2 from dolichyl pyrophosphate to acceptor sites on the target protein by an oligosaccharyltransferase complex. This core oligosaccharide is sequentially processed by several ER glycosidases and by an endomannosidase (E.C. 3.2.1.130), such as MANEA, in the Golgi. MANEA catalyzes the release of mono-, di-, and triglucosylmannose oligosaccharides by cleaving the alpha-1,2-mannosidic bond that links them to high-mannose glycans (Hamilton et al., 2005 [PubMed 15677381]).[supplied by OMIM, Sep 2008]

Canonical amino-acid sequenceUniProt

462 residues, UniProt reviewed canonical sequence.

>Q5SRI9|MANEA
     1  MAKFRRRTCI ILALFILFIF SLMMGLKMLR PNTATFGAPF GLDLLPELHQ RTIHLGKNFD
    61  FQKSDRINSE TNTKNLKSVE ITMKPSKASE LNLDELPPLN NYLHVFYYSW YGNPQFDGKY
   121  IHWNHPVLEH WDPRIAKNYP QGRHNPPDDI GSSFYPELGS YSSRDPSVIE THMRQMRSAS
   181  IGVLALSWYP PDVNDENGEP TDNLVPTILD KAHKYNLKVT FHIEPYSNRD DQNMYKNVKY
   241  IIDKYGNHPA FYRYKTKTGN ALPMFYVYDS YITKPEKWAN LLTTSGSRSI RNSPYDGLFI
   301  ALLVEEKHKY DILQSGFDGI YTYFATNGFT YGSSHQNWAS LKLFCDKYNL IFIPSVGPGY
   361  IDTSIRPWNT QNTRNRINGK YYEIGLSAAL QTRPSLISIT SFNEWHEGTQ IEKAVPKRTS
   421  NTVYLDYRPH KPGLYLELTR KWSEKYSKER ATYALDRQLP VS

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against MANEA can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability
Other membrane
Secreted
No
Transmembrane segments
1
Mean surface accessibility (rSASA)
0.31
Highest tissue expression
21 nTPM

Expression across tissuesHPA

Tissue

  • liver: 21 nTPM
  • kidney: 11 nTPM
  • adipose tissue: 10 nTPM
  • parathyroid gland: 10 nTPM
  • stomach: 10 nTPM
  • thyroid gland: 9.8 nTPM

Single-cell type

  • plasma cells: 130 nCPM
  • hepatocytes: 69 nCPM
  • corticotrophs: 42 nCPM
  • gastric chief cells: 36 nCPM
  • podocytes: 34 nCPM
  • mucous neck cells: 29 nCPM

Immune cell

  • basophil: 5.9 nTPM
  • T-reg: 3.6 nTPM
  • intermediate monocyte: 2.9 nTPM
  • MAIT T-cell: 2.7 nTPM
  • non-classical monocyte: 2.6 nTPM
  • memory B-cell: 2.5 nTPM

Brain region

  • white matter: 10 nTPM
  • medulla oblongata: 8.7 nTPM
  • spinal cord: 7.7 nTPM
  • hypothalamus: 7.6 nTPM
  • cerebellum: 7.1 nTPM
  • thalamus: 6.9 nTPM

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)
0.87
gnomAD pLI
0
gnomAD missense Z
-0.01
DepMap mean gene effect
0.08
DepMap dependency class
selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Molecular functions

Cellular components

Protein domainsUniProt · Pfam · InterPro

KeywordsUniProt

Antibody and autoantibody relevanceSeroatlas analysis

Seroatlas reads MANEA as an antibody target. Whether an autoantibody or antibody against MANEA could matter depends on whether native MANEA is physically reachable, whether the body needs it intact, and whether it acts in a disease-relevant tissue.

MANEA is annotated as predominantly intracellular. Intracellular proteins are common autoantibody markers, becoming visible to the immune system after cell injury or altered processing, but are usually markers of disease rather than direct drivers.

Annotation status

The present source text does not explicitly label MANEA as an autoantigen. Seroatlas presents hypothesis context only and does not manufacture a known-serology claim.

Canonical record: https://seroatlas.com/gene/MANEA. Study-independent annotations aggregated from UniProt, Human Protein Atlas, PubMed, IEDB, Pfam, InterPro, Gene Ontology, AlphaFold, gnomAD, DepMap, ClinVar, TCGA. Catalog release seroatlas-reviewed-human-uniprot-20260313.

Seroatlas is the reference for exploring autoantibody and antibody serology at the human-protein level: the autoreactome and human serome, multiplex serology (HuProt, HuScan, VirScan, PhIP-Seq).

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