LGMN

Protein identityUniProt · HPA

UniProt accession

Q99538

Gene

LGMN

Ensembl

ENSG00000100600

Chromosome

14

Canonical length

433 aa

Protein class

Cancer-related genes, Enzymes, Metabolic proteins, Predicted intracellular proteins

Subcellular location

Nucleoplasm,Vesicles

Secretome location

Intracellular and membrane

Quaternary structure

Homodimer

OverviewNCBI Gene

This gene encodes a cysteine protease that has a strict specificity for hydrolysis of asparaginyl bonds. This enzyme may be involved in the processing of bacterial peptides and endogenous proteins for MHC class II presentation in the lysosomal/endosomal systems. Enzyme activation is triggered by acidic pH and appears to be autocatalytic. Protein expression occurs after monocytes differentiate into dendritic cells. A fully mature, active enzyme is produced following lipopolysaccharide expression in mature dendritic cells. Overexpression of this gene may be associated with the majority of solid tumor types. This gene has a pseudogene on chromosome 13. Several alternatively spliced transcript variants have been described, but the biological validity of only two has been determined. These two variants encode the same isoform. [provided by RefSeq, Jul 2008]

Canonical amino-acid sequenceUniProt

433 residues, UniProt reviewed canonical sequence.

>Q99538|LGMN
     1  MVWKVAVFLS VALGIGAVPI DDPEDGGKHW VVIVAGSNGW YNYRHQADAC HAYQIIHRNG
    61  IPDEQIVVMM YDDIAYSEDN PTPGIVINRP NGTDVYQGVP KDYTGEDVTP QNFLAVLRGD
   121  AEAVKGIGSG KVLKSGPQDH VFIYFTDHGS TGILVFPNED LHVKDLNETI HYMYKHKMYR
   181  KMVFYIEACE SGSMMNHLPD NINVYATTAA NPRESSYACY YDEKRSTYLG DWYSVNWMED
   241  SDVEDLTKET LHKQYHLVKS HTNTSHVMQY GNKTISTMKV MQFQGMKRKA SSPVPLPPVT
   301  HLDLTPSPDV PLTIMKRKLM NTNDLEESRQ LTEEIQRHLD ARHLIEKSVR KIVSLLAASE
   361  AEVEQLLSER APLTGHSCYP EALLHFRTHC FNWHSPTYEY ALRHLYVLVN LCEKPYPLHR
   421  IKLSMDHVCL GHY

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against LGMN can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Intracellular

Secreted

No

Transmembrane segments

0

Mean surface accessibility (rSASA)

0.23

Highest tissue expression

246 nTPM

Expression across tissuesHPA

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.69

gnomAD pLI

0

gnomAD missense Z

1.22

DepMap mean gene effect

0.11

DepMap dependency class

none

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 4% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
• antigen processing and presentation of exogenous peptide antigen via MHC class II
• associative learning
• cellular response to amyloid-beta
• cellular response to calcium ion
• cellular response to hepatocyte growth factor stimulus
• dendritic spine organization
• memory
• negative regulation of ERBB signaling pathway
• negative regulation of gene expression
• negative regulation of multicellular organism growth
• negative regulation of neuron apoptotic process
• positive regulation of cell population proliferation
• positive regulation of endothelial cell chemotaxis
• positive regulation of long-term synaptic potentiation
• positive regulation of mitotic cell cycle
• positive regulation of monocyte chemotaxis
• protein maturation
• proteolysis
• proteolysis involved in protein catabolic process
• receptor catabolic process
• renal system process
• response to acidic pH
• vacuolar protein processing
• vitamin D metabolic process

Molecular functions

• cysteine-type endopeptidase activity
• endopeptidase activator activity
• peptidase activity
• tau protein binding

Cellular components

• apical part of cell
• cytoplasm
• endolysosome lumen
• extracellular exosome
• extracellular region
• late endosome
• lysosomal lumen
• lysosome
• perinuclear region of cytoplasm

Protein domainsUniProt · Pfam · InterPro

• Peptidase C13, legumain
• Peptidase C13 family
• Asparaginyl endopeptidase
• Legumain prodomain superfamily
• Legumain, prodomain
• Legumain, prodomain

KeywordsUniProt

• Disulfide bond
• Glycoprotein
• Hydrolase
• Lysosome
• Protease
• Signal
• Thiol protease
• Zymogen

InteractionsUniProt · HPA

Protein binding partners of LGMN in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• FBXO6