ITGAM

Protein identityUniProt · HPA

UniProt accession

P11215

Gene

ITGAM

Ensembl

ENSG00000169896

Chromosome

16

Canonical length

1152 aa

Protein class

Cancer-related genes, CD markers, Disease related genes, Predicted intracellular proteins, Predicted membrane proteins

OverviewNCBI Gene

This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

Canonical amino-acid sequenceUniProt

1152 residues, UniProt reviewed canonical sequence.

>P11215|ITGAM
     1  MALRVLLLTA LTLCHGFNLD TENAMTFQEN ARGFGQSVVQ LQGSRVVVGA PQEIVAANQR
    61  GSLYQCDYST GSCEPIRLQV PVEAVNMSLG LSLAATTSPP QLLACGPTVH QTCSENTYVK
   121  GLCFLFGSNL RQQPQKFPEA LRGCPQEDSD IAFLIDGSGS IIPHDFRRMK EFVSTVMEQL
   181  KKSKTLFSLM QYSEEFRIHF TFKEFQNNPN PRSLVKPITQ LLGRTHTATG IRKVVRELFN
   241  ITNGARKNAF KILVVITDGE KFGDPLGYED VIPEADREGV IRYVIGVGDA FRSEKSRQEL
   301  NTIASKPPRD HVFQVNNFEA LKTIQNQLRE KIFAIEGTQT GSSSSFEHEM SQEGFSAAIT
   361  SNGPLLSTVG SYDWAGGVFL YTSKEKSTFI NMTRVDSDMN DAYLGYAAAI ILRNRVQSLV
   421  LGAPRYQHIG LVAMFRQNTG MWESNANVKG TQIGAYFGAS LCSVDVDSNG STDLVLIGAP
   481  HYYEQTRGGQ VSVCPLPRGR ARWQCDAVLY GEQGQPWGRF GAALTVLGDV NGDKLTDVAI
   541  GAPGEEDNRG AVYLFHGTSG SGISPSHSQR IAGSKLSPRL QYFGQSLSGG QDLTMDGLVD
   601  LTVGAQGHVL LLRSQPVLRV KAIMEFNPRE VARNVFECND QVVKGKEAGE VRVCLHVQKS
   661  TRDRLREGQI QSVVTYDLAL DSGRPHSRAV FNETKNSTRR QTQVLGLTQT CETLKLQLPN
   721  CIEDPVSPIV LRLNFSLVGT PLSAFGNLRP VLAEDAQRLF TALFPFEKNC GNDNICQDDL
   781  SITFSFMSLD CLVVGGPREF NVTVTVRNDG EDSYRTQVTF FFPLDLSYRK VSTLQNQRSQ
   841  RSWRLACESA SSTEVSGALK STSCSINHPI FPENSEVTFN ITFDVDSKAS LGNKLLLKAN
   901  VTSENNMPRT NKTEFQLELP VKYAVYMVVT SHGVSTKYLN FTASENTSRV MQHQYQVSNL
   961  GQRSLPISLV FLVPVRLNQT VIWDRPQVTF SENLSSTCHT KERLPSHSDF LAELRKAPVV
  1021  NCSIAVCQRI QCDIPFFGIQ EEFNATLKGN LSFDWYIKTS HNHLLIVSTA EILFNDSVFT
  1081  LLPGQGAFVR SQTETKVEPF EVPNPLPLIV GSSVGGLLLL ALITAALYKL GFFKRQYKDM
  1141  MSEGGPPGAE PQ

LocalizationUniProt · AlphaFold · HPA

Whether an antibody against ITGAM can act on the native protein depends on physical access: surface and secreted proteins are reachable by circulating antibodies, intracellular proteins usually are not.

Antibody reachability

Cell surface

Secreted

No

Transmembrane segments

1

Mean surface accessibility (rSASA)

0.27

Highest tissue expression

173 nTPM

Expression across tissuesHPA

DiseaseUniProt · ClinVar · IEDB · PubMed

Four sources answering four different questions about ITGAM.

Disease | AllUniProt

Conditions ITGAM is implicated in, by any mechanism.

• Systemic lupus erythematosus 6 (SLEB6) MIM:609939

ReferencesPubMed · IEDB

Publications for ITGAM from three distinct lines of evidence, kept separate because they answer different questions: whether antibodies are directed at the protein, whether a B-cell epitope has been mapped on it, and whether a T-cell epitope has. Each is labelled with its source.

Sources: PubMed — antigen-level antibody evidence from a custom retrieval. Records matching a controlled set of autoantibody terms (the MeSH descriptors Autoantibodies and Autoantigens, with title and abstract term variants) were obtained through NCBI E-utilities, and their titles and abstracts parsed for constructions that direct an antibody at a named protein rather than for co-occurrence. Captured names were resolved against UniProt nomenclature and each antigen adjudicated individually against the source text. IEDB — curated epitope assays from the Immune Epitope Database (Vita et al., Nucleic Acids Research 2019). Bibliographic records from PubMed and MeSH, U.S. National Library of Medicine; citation metrics from NIH iCite (Hutchins et al., PLoS Biology 2016). Titles link to PubMed; abstracts are not reproduced here. The NLM does not endorse this analysis.

Genetic constraint and essentialitygnomAD · DepMap

Does the body need this protein intact? Low LOEUF or a strong DepMap dependency means loss or blockade of the protein is likely to be felt.

gnomAD LOEUF (loss-of-function intolerance)

0.64

gnomAD pLI

0

gnomAD missense Z

1.42

DepMap mean gene effect

0.04

DepMap dependency class

selective

Cancer expressionTCGA

Across TCGA tumor cohorts, this protein is over-expressed in roughly 3% of surveyed tumor types (aggregate summary; per-cohort expression, alteration, and survival load in the interactive view).

OntologyGO

Biological processes

• amyloid-beta clearance
• cell adhesion
• cell-cell adhesion
• cell-cell adhesion via plasma-membrane adhesion molecules
• cell-matrix adhesion
• complement receptor mediated signaling pathway
• complement-mediated synapse pruning
• ectodermal cell differentiation
• forebrain development
• heterotypic cell-cell adhesion
• innate immune response
• integrin-mediated signaling pathway
• microglial cell activation
• negative regulation of dopamine metabolic process
• phagocytosis, engulfment
• positive regulation of microglial cell mediated cytotoxicity
• positive regulation of neutrophil degranulation
• positive regulation of protein targeting to membrane
• positive regulation of superoxide anion generation
• receptor-mediated endocytosis
• response to amphetamine
• response to curcumin
• response to estradiol
• response to Gram-positive bacterium
• response to ischemia
• response to mechanical stimulus
• vertebrate eye-specific patterning

Molecular functions

• amyloid-beta binding
• cargo receptor activity
• complement component C3b binding
• heat shock protein binding
• integrin binding
• metal ion binding
• signaling receptor activity

Cellular components

• cell surface
• external side of plasma membrane
• extracellular exosome
• extracellular space
• integrin alphaM-beta2 complex
• integrin complex
• plasma membrane
• plasma membrane raft
• specific granule membrane
• tertiary granule membrane

Protein domainsUniProt · Pfam · InterPro

• Integrin alpha chain
• von Willebrand factor, type A
• FG-GAP repeat
• Integrin alpha beta-propellor
• Integrin alpha, first immunoglubulin-like domain
• Integrin alpha chain, C-terminal cytoplasmic region, conserved site
• Integrin alpha, N-terminal
• Integrin domain superfamily
• von Willebrand factor A-like domain superfamily
• Integrin alpha, second immunoglobulin-like domain
• Integrin alpha-X-like, third Ig-like domain
• von Willebrand factor type A domain
• Integrin alpha cytoplasmic region
• FG-GAP repeat
• Integrin alpha Ig-like domain 1
• Integrin alpha Ig-like domain 2
• Integrin alpha-X-like, Ig-like domain 3

KeywordsUniProt

• Calcium
• Cell adhesion
• Cell membrane
• Disulfide bond
• Glycoprotein
• Immunity
• Innate immunity
• Integrin
• Magnesium
• Membrane
• Metal-binding
• Receptor
• Repeat
• Signal
• Systemic lupus erythematosus
• Transmembrane
• Transmembrane helix

InteractionsUniProt · HPA

Protein binding partners of ITGAM in the human serome: UniProt-annotated complex subunits plus reported interactors. Each links to its own Seroatlas record.

• JAM3
• THBD
• TMEM268
• AUP1